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H2DCFDA

Cat. No. 5935 2 篇引用文献 2 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Fluorescent ROS indicator; cell permeable
化学名： 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
纯度： ≥95% (HPLC)
说明书
引用文献 (2)
评论 (2)
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for H2DCFDA

H2DCFDA is a fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.

技术数据 for H2DCFDA

分子量 487.29
公式 C24H16Cl2O7
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 4091-99-0
PubChem ID 77718
InChI Key PXEZTIWVRVSYOK-UHFFFAOYSA-N
Smiles ClC1=CC(C(C2=CC=CC=C2C(O)=O)C(C=C(Cl)C(OC(C)=O)=C3)=C3O4)=C4C=C1OC(C)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for H2DCFDA

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 48.73 100
ethanol 4.87 10

制备储备液 for H2DCFDA

以下数据基于产品分子量 487.29。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 2.05 mL 10.26 mL 20.52 mL
5 mM 0.41 mL 2.05 mL 4.1 mL
10 mM 0.21 mL 1.03 mL 2.05 mL
50 mM 0.04 mL 0.21 mL 0.41 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Dilution Calculator

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产品说明书 for H2DCFDA

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for H2DCFDA

参考文献是支持产品生物活性的出版物。

Nicholson et al (2013) Sigma-2 receptors play a role in cellular metabolism: stimulation of glycolytic hallmarks by CM764 in human SK-N-SH neuroblastoma. J.Pharmacol.Exp.Ther. 356 232 PMID: 26574517

Santabárbara-Ruiz et al (2015) ROS-induced JNK and p38 signaling is required for unpaired cytokine activation during drosophila regeneration. PLoS Genet. 11 e1005595 PMID: 26496642

If you know of a relevant reference for H2DCFDA, please let us know.

按标靶查看相关产品

关键词: H2DCFDA, H2DCFDA supplier, Cell, permeable, fluorescent, probes, ROS, reactive, oxygen, species, indicators, dyes, Fluorescent, Indicators, and, Sensors, Donors, Flow, Cytometry, 5935, Tocris Bioscience

2 篇 H2DCFDA 的引用文献

引用文献是使用了 Tocris 产品的出版物。 H2DCFDA 的部分引用包括：

Walter F et al (2022) CRISPR-Cas9 screen identifies oxidative phosphorylation as essential for cancer cell survival at low extracellular pH. Cell Rep 38 110493 PMID: 35263578

Andris et al (2022) Oxidative stress from DGAT1 oncoprotein inhibition in melanoma suppresses tumor growth when ROS defenses are also breached. Cell Rep 39 110995 PMID: 35732120

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H2DCFDA 的评论

平均评分: 5 (基于 2 条评论。)

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H2DCFDA detects intracellular ROS levels in 3D Glioblastoma cell lines.
By sara Azeem on 01/08/2020
分析类型： In Vitro
种属： Human
细胞系/组织： G7

Glioblatsoma cell line G7 was cultured in 3D sphere model and the levels of intracellular ROS were measured.

Try different dilutions to get the one best suited to you.We treated the cells for 30 minutes and that was enough to get robust results

review image
H2DCFDA detects intracellular ROS levels in 15d-PGJ2 treated cardiomyocytes.
By Chintan Koyani on 10/13/2018
分析类型： In Vitro
种属： Mouse
细胞系/组织： HL-1 cardiomyocytes

HL-1 cardiomyocytes were stimulated with 15d-PGJ2 up to 60 min to follow intracellular ROS levels using H2DCFDA.

review image

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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