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说明： GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy
化学名： 4-Cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]benzeneacetonitrile
纯度： ≥98% (HPLC)
生物活性 for HBC 530
HBC 530 is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum = 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum = 475 - 575 nm.
To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).
Optical Data for HBC 530
| λabs | 485 nm |
|---|---|
| λem | 530 nm |
| Extinction Coefficient (ε) | 65300 M-1cm-1 |
| Quantum Yield (φ) | 0.66 |
| Closest Laser line | 488 nm |
| Application | Fluorogen, used with RNA Light-up Aptamer, Confocal Microscopy |
| Alternative For | Cat. No. 6434 |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for HBC 530
| 分子量 | 303.37 |
| 公式 | C19H17N3O |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 156840-13-0 |
| InChI Key | DIAVZHWDYXQAFC-LDADJPATSA-N |
| Smiles | CN(CCO)C1=CC=C(C=C1)/C=C(C#N)/C2=CC=C(C=C2)C#N |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for HBC 530
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 30.34 | 100 |
制备储备液 for HBC 530
以下数据基于产品分子量 303.37。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.3 mL | 16.48 mL | 32.96 mL |
| 5 mM | 0.66 mL | 3.3 mL | 6.59 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.3 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
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产品说明书 for HBC 530
参考文献 for HBC 530
参考文献是支持产品生物活性的出版物。
Chen et al (2019) Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 37 1287 PMID: 31548726
If you know of a relevant reference for HBC 530, please let us know.
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关键词: HBC 530, HBC 530 supplier, HBC530, pepper, aptamer, fluorophore, pepper530, RNA, imaging, live, cells, confocal, light, up, FLAPs, RMFPs, Aptamer-based, Imaging, Fluorescent, Probes, 7277, Tocris Bioscience
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该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals