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说明： Fluorescent Ca2+ indicator
化学名： 2-[4-[Bis[2-[(acetoxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid (acetoxy)methyl ester
纯度： ≥90% (HPLC)
生物活性 for Indo 1AM
Indo 1AM is a fluorescent Ca2+ indicator (Kd for Ca2+ = 250 nM). Cell-permeable. Displays ratiometric emission wavelength change upon Ca2+ binding. Excitation/emission λ are 355/475 nm for no Ca2+ and 355/401 nm for high Ca2+. Absorption maxima are 349 and 331 nm for free anion and Ca complex, respectively. Respective emission maxima are 485 and 410 nm. Quantum yield ~0.5. Ideal for flow cytometry..
This product is typically prepared in DMSO.
Optical Data for Indo 1AM
| Emission Color | Blue |
|---|---|
| λabs | 355 nm |
| λem | 474 nm |
| Closest Laser line | 350 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for Indo 1AM
| 分子量 | 1009.91 |
| 公式 | C47H51N3O22 |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 112926-02-0 |
| PubChem ID | 123918 |
| InChI Key | CAWBRCOBJNWRLK-UHFFFAOYSA-N |
| Smiles | CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=C(OCCOC2=C(C=CC(=C2)C2=CC3=C(N2)C=C(C=C3)C(=O)OCOC(C)=O)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=C(C)C=C1 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for Indo 1AM
参考文献 for Indo 1AM
参考文献是支持产品生物活性的出版物。
Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314
If you know of a relevant reference for Indo 1AM, please let us know.
关键词: Indo 1AM, Indo 1AM supplier, Indo1AM, fluorescent, calcium, Ca2+, indicator, flow, cytometry, cell, permeable, Ion, Indicators, Fluorescent, 6704, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals