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说明： Fluorescent sodium ion (Na+) indicator, membrane permeable
化学名： Bis(acetoxymethyl) 3,3'-(6-(acetoxymethoxy)-4,5-dichloro-9-(3-methoxy-4-(13-(2-methoxy-4-methylphenyl)-1,4,10-trioxa-7,13-diazacyclopentadecan-7-yl)phenyl)-3-oxo-3H-xanthene-2,7-diyl)dipropionate
纯度： ≥90% (HPLC)
生物活性 for ING-2 AM
ING-2 AM is a membrane-permeable fluorescent sodium ion (Na+) indicator. It binds to sodium (Kd = 20 mM). Excitation and emission maxima (λ) are 525 and 545 nm, respectively. It displays 20-fold selectivity over K+ ions. It is used in in vitro and in situ to detect real-time sodium influx.
It is recommended to prepare stock solutions in DMSO.
Optical Data for ING-2 AM
| λabs | 525 nm |
|---|---|
| λem | 545 nm |
| Cell Permeable | Yes |
| Application | Confocal microscopy, Flow cytometry, Two-photon microscopy |
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技术数据 for ING-2 AM
| 分子量 | 1083.96 |
| 公式 | C53H60Cl2N2O18 |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 1642554-49-1 |
| PubChem ID | 163341954 |
| InChI Key | OTIAVQNFWAJQKZ-UHFFFAOYSA-N |
| Smiles | O=C(C)OCOC(CCC1=CC2=C(C3=CC(OC)=C(N4CCOCCOCCN(CCOCC4)C5=CC=C(C)C=C5OC)C=C3)C6=C(C(Cl)=C(C(CCC(OCOC(C)=O)=O)=C6)OCOC(C)=O)OC2=C(C1=O)Cl)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for ING-2 AM
参考文献 for ING-2 AM
参考文献是支持产品生物活性的出版物。
Gregoriades et al (2019) Genetic and pharmacological inactivation of apical Na+-K+-2Cl- cotransporter 1 in choroid plexus epithelial cells reveals the physiological function of the cotransporter. Am.J.Physiol.Cell Physiol. 316 C525 PMID: 30576237
Blömer et al (2021) Ultrafast sodium imaging of the axon initial segment of neurons in mouse brain slices. Curr.Protoc. 1 e64 PMID: 33657273
Naumann et al (2018) Photophysical properties of Na+ -indicator dyes suitable for quantitative two-photon fluorescence-lifetime measurements. J.Microsc. 272 136 PMID: 30191999
Roder et al (2014) ANG-2 for quantitative Na(+) determination in living cells by time-resolved fluorescence microscopy. Photochem.Photobiol.Sci. 13 1699 PMID: 25311309
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Fluorescent Dyes and Probes Research Product Guide
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