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说明： Fluorescent BET bromodomain probe; suitable for use in TR-FRET
化学名： (S)-5-(3-(1-(4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)thioureido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid trifluoroacetate
纯度： ≥95% (HPLC)
生物活性 for JQ1-FITC
JQ1-FITC is a fluorescent BET bromodomain probe. It binds with high affinity to BRD4(BD1) and BRD4(BD2) (KD,app values are 6.5 nM and 5.8 nM, respectively, using GST-tagged BRD4 proteins measured by CoraFluor™ TR-FRET assay; 58.7 nM for BRD4(BD1) in a fluorescence anisotropy assay). Excitation and emission maxima (λ) are 495 nm and 525 nm.
Optical Data for JQ1-FITC
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 525 nm |
| Extinction Coefficient (ε) | 7500 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Application | TR-FRET, Fluorescence polarization assays |
| Alternative For | FITC |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for JQ1-FITC
| 分子量 | 1106.58 |
| 公式 | C50H50ClN7O9S2.CF3CO2H |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| InChI Key | QRVVCZSOSIMDNN-LMDYQTDMSA-N |
| Smiles | O=C(NCCCOCCOCCOCCCNC(NC1=CC=C(C(C(O)=O)=C1)C2=C(C(OC3=C2C=CC(O)=C3)=C4)C=CC4=O)=S)C[C@@H]5N=C(C6=CC=C(Cl)C=C6)C7=C(SC(C)=C7C)N8C5=NN=C8C.OC(C(F)(F)F)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for JQ1-FITC
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 11.07 | 10 |
制备储备液 for JQ1-FITC
以下数据基于产品分子量 1106.58。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 9.04 mL | 45.18 mL | 90.37 mL |
| 0.5 mM | 1.81 mL | 9.04 mL | 18.07 mL |
| 1 mM | 0.9 mL | 4.52 mL | 9.04 mL |
| 5 mM | 0.18 mL | 0.9 mL | 1.81 mL |
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产品说明书 for JQ1-FITC
参考文献 for JQ1-FITC
参考文献是支持产品生物活性的出版物。
Payne et al (2022) A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 18 1333 PMID: 35649410
If you know of a relevant reference for JQ1-FITC, please let us know.
按标靶查看相关产品
关键词: JQ1-FITC, JQ1-FITC supplier, JQ1, FITC, fluorescent, probes, time-resolved, fluorescence, resonance, energy, transfer, TR-FRET, cereblon, E3, ligases, and, FP, Assay, Reagents, Other, Fluorescent, Probes, Bromodomains, 7722, Tocris Bioscience
1 篇 JQ1-FITC 的引用文献
引用文献是使用了 Tocris 产品的出版物。 JQ1-FITC 的部分引用包括：
Payne et al (2024) Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology. STAR Protoc. 5 103129 PMID: 38857155
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