MitoBrilliant™ 646

Name: MitoBrilliant™ 646
Brand: Tocris Bioscience
SKU: 7700
Rating: 5 (1 reviews)

Cat. No. 7700 1 条评论提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Universal red fluorescent mitochondrial stain for both live and fixed cells

纯度： ≥95% (HPLC)

说明书

引用文献

实验方案 (1)

文献 (2)

生物活性 Scientific Data 技术数据溶解性计算器说明书参考文献

生物活性 for MitoBrilliant™ 646

MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor^® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.

Key information: MitoBrilliant^™ 646 is a red-fluorescent mitochondrial stain for both live and fixed cells.

Used for: mitochondrial staining for both live and pre-fixed cells/tissue.

Application: flow cytometry, super-resolution microscopy-STED, high content screening, IHC/ICC.

Properties and Photophysical Data: MitoBrilliant^™ 646 is retained in mitochondria following fixation with exceptionally clear staining and is retained in the mitochondria of live cells following loss of the mitochondrial membrane potential. Excitation and emission maxima (λ) are 655 nm and 668 nm, respectively; extinction coefficient = 125,000 M^-1cm^-1.

Two dyes that accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner are also available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).

Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.

MitoBrilliant is a trademark of Bio-Techne Corp.

Scientific Data

Live-cell Imaging

High resolution confocal image of MitoBrilliant 646 shows the morphology of mitochondria in live cell

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Live-cell image of mitochondria stained with MitoBrilliant™ 646 HeLa cells were incubated with MitoBrilliant™ 646 (100 nM) for 40 minutes and counterstained with DAPI (Catalog # 5748). Image was taken using an LSM880 Confocal using a 63x objective. Scale bar = 10 µm.

Fixed-cell Imaging

High resolution confocal image of MitoBrillant 646 shows the morphology of mitochondria in fixed-cells

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Fixed-cell image of mitochondria stained with MitoBrilliant™ 646. Dermal fibroblast cells were incubated with MitoBrilliant™ 646 (100 nM) for 1 hour at 37°C, fixed with ice-cold acetone-methanol (1:1) and counterstained with Hoechst 33342 (Catalog # 5117). Image was taken using an Echo Revolve microscope using a 20x objective. Scale bar = 20 µm.

Product Name	Core dye structure	Abs/Em (nm)	Δψ_m dependent	Live/Fixed cell use	Stain pre-fixed cells?	Image without wash step	Demonstrated Applications
MitoBrilliant™ 646 Cat. No. 7700	Janelia Fluor® technology	655/668	No*	Suitable for both live and fixed cell work	Yes	Yes, but replacing media recommended	Fixed-cell imaging, Live-cell imaging, Flow Cytometry, IHC/ICC, Super-resolution microscopy – STED, High-content screening
MitoBrilliant™ Live 646 Cat. No. 7417	Janelia Fluor® technology	648/662	Yes	Live-cell work only	No	Yes, but replacing media recommended	Live-cell imaging, Flow Cytometry, High-content screening
MitoBrilliant™ Live 549 Cat. No. 7693	Janelia Fluor® technology	550/568	Yes	Live-cell work only	No	Yes, but replacing media recommended	Live-cell imaging, Flow Cytometry, High-content screening

Optical Data for MitoBrilliant™ 646

Emission Color	Red
λ_abs	655 nm
λ_em	668 nm
Extinction Coefficient (ε)	125000 M^-1cm^-1
Closest Laser line	640 nm
Application	Fixed-cell imaging, Live-cell imaging, Flow Cytometry, IHC/ICC, Super-resolution microscopy – STED,

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for MitoBrilliant™ 646

分子量	493.55
储存	Store at -20°C
纯度	≥95% (HPLC)

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for MitoBrilliant™ 646

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	4.94	10

制备储备液 for MitoBrilliant™ 646

以下数据基于产品分子量 493.55。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.1 mM	20.26 mL	101.31 mL	202.61 mL
0.5 mM	4.05 mL	20.26 mL	40.52 mL
1 mM	2.03 mL	10.13 mL	20.26 mL
5 mM	0.41 mL	2.03 mL	4.05 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

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产品说明书 for MitoBrilliant™ 646

分析证书/产品说明书

化学品安全技术说明书

参考文献 for MitoBrilliant™ 646

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关键词: MitoBrilliant 646, MitoBrilliant 646 supplier, MitoBrilliant, red, fluorescent, mitochondrial, marker, stain, mitotracker, mitochondria, Flow, Cytometry, Mitobrilliant, 7700, Tocris Bioscience

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MitoBrilliant™ 646 produced excellent results!.

By Jessica Cale on 11/20/2023

种属： Human

Fixed-cell image of mitochondria stained with MitoBrilliant™ 646:Dermal fibroblast cells were incubated with MitoBrilliant™ 646 (100 nM) for 1 hour at 37°C, fixed with ice-cold acetone-methanol (1:1) and counterstained with Hoechst 33342. Image was taken using an Echo Revolve microscope using a 20x objective. Scale bar = 20 µm.

MitoBrilliant™ 646 produced excellent results as both a live and fixed cell stain. As a live cell stain MitoBrilliant™ 646 produced a stable signal within 20 minutes. For use as fixed-cell stain optimal conditions were found to be a 1-hour incubation in live cells followed by fixation with ice-cold acetone-methanol.

MitoBrilliant™ 646 实验方案

以下实验方案包含使用 MitoBrilliant™ 646（目录编号 7700）的更多信息。

MitoBrilliant™ Protocol

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

MitoBrilliant Research Product Guide

This product guide provides a background, protocols and data from use in different research applications for our Mitobrilliant™ fluorescent mitochondrial probes:

MitoBrilliant™ 646
MitoBrilliant™ Live 646
MitoBrilliant™ Live 549

Novel Live-cell and Fixable Mitochondrial Probes Poster

Fluorescent probes that accumulate in the mitochondria are useful tools to explore mitochondrial function and cell health. This poster reports the development inhouse and characterization of novel fluorescent probes, known as MitoBrilliant that specifically localize to mitochondria due to mitochondrial membrane potential. The probes are suitable for live or fixed cell staining. The data demonstrate the specificity and enhanced properties of our MitoBrilliant dyes and their use in a range of applications. This poster was presented at CYTO 2022, June 2022, Philadelphia, USA.