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Description: Orange fluorescent mitochondrial stain for live cells, Δψm dependent
Purity: ≥95% (HPLC)
Biological Activity for MitoBrilliant™ Live 549
MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.
Key information: MitoBrilliant® Live 549 is an orange-fluorescent mitochondrial stain for live cells, Δψm dependent.
Used for: mitochondrial staining for live cells.
Applications: flow cytometry, high content screening.
Properties and Photophysical Data: the MitoBrilliant® Live dyes accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner. Upon loss of the mitochondrial membrane potential, the dyes disperse, and so provide a dynamic assessment of the mitochondrial membrane potential in live cells. Excitation and emission maxima (λ) are 550 nm and 568 nm, respectively, extinction coefficient = 100,000 M-1cm-1.
Two Live dyes are available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).
A universal stain for mitochondria in both live and fixed cells is also available: MitoBrilliant™ 646.
Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.
MitoBrilliant is a trademark of Bio-Techne Corp.
| Product Name | Core dye structure | Abs/Em (nm) | Δψm dependent | Live/Fixed cell use | Stain pre-fixed cells? | Image without wash step | Demonstrated Applications | MitoBrilliant™ 646 Cat. No. 7700 | Janelia Fluor® technology | 655/668 | No* | Suitable for both live and fixed cell work | Yes | Yes, but replacing media recommended | Fixed-cell imaging, Live-cell imaging, Flow Cytometry, IHC/ICC, Super-resolution microscopy – STED, High-content screening |
|---|---|---|---|---|---|---|---|
| MitoBrilliant™ Live 646 Cat. No. 7417 | Janelia Fluor® technology | 648/662 | Yes | Live-cell work only | No | Yes, but replacing media recommended | Live-cell imaging, Flow Cytometry, High-content screening |
| MitoBrilliant™ Live 549 Cat. No. 7693 | Janelia Fluor® technology | 550/568 | Yes | Live-cell work only | No | Yes, but replacing media recommended | Live-cell imaging, Flow Cytometry, High-content screening |
Optical Data for MitoBrilliant™ Live 549
| Emission Color | Yellow/Orange |
|---|---|
| λabs | 550 nm |
| λem | 568 nm |
| Extinction Coefficient (ε) | 100000 M-1cm-1 |
| Closest Laser line | 561 nm |
| Application | Live-cell Imaging, Flow Cytometry, High-content Screening |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for MitoBrilliant™ Live 549
| M. Wt | 416.95 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for MitoBrilliant™ Live 549
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 20.85 | 50 |
Preparing Stock Solutions for MitoBrilliant™ Live 549
The following data is based on the product molecular weight 416.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 4.8 mL | 23.98 mL | 47.97 mL |
| 2.5 mM | 0.96 mL | 4.8 mL | 9.59 mL |
| 5 mM | 0.48 mL | 2.4 mL | 4.8 mL |
| 25 mM | 0.1 mL | 0.48 mL | 0.96 mL |
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Product Datasheets for MitoBrilliant™ Live 549
References for MitoBrilliant™ Live 549
References are publications that support the biological activity of the product.
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Citations for MitoBrilliant™ Live 549
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Protocols for MitoBrilliant™ Live 549
The following protocol features additional information for the use of MitoBrilliant™ Live 549 (Cat. No. 7693).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
MitoBrilliant Research Product Guide
This product guide provides a background, protocols and data from use in different research applications for our Mitobrilliant™ fluorescent mitochondrial probes:
- MitoBrilliant™ 646
- MitoBrilliant™ Live 646
- MitoBrilliant™ Live 549
