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Submit ReviewMitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.
Key information: MitoBrilliant® Live 549 is an orange-fluorescent mitochondrial stain for live cells, Δψm dependent.
Used for: mitochondrial staining for live cells.
Applications: flow cytometry, high content screening.
Properties and Photophysical Data: the MitoBrilliant® Live dyes accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner. Upon loss of the mitochondrial membrane potential, the dyes disperse, and so provide a dynamic assessment of the mitochondrial membrane potential in live cells. Excitation and emission maxima (λ) are 550 nm and 568 nm, respectively, extinction coefficient = 100,000 M-1cm-1.
Two Live dyes are available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).
A universal stain for mitochondria in both live and fixed cells is also available: MitoBrilliant™ 646.
Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.
MitoBrilliant is a trademark of Bio-Techne Corp.
Product Name | Core dye structure | Abs/Em (nm) | Δψm dependent | Live/Fixed cell use | Stain pre-fixed cells? | Image without wash step | Demonstrated Applications | MitoBrilliant™ 646 Cat. No. 7700 | Janelia Fluor® technology | 655/668 | No* | Suitable for both live and fixed cell work | Yes | Yes, but replacing media recommended | Fixed-cell imaging, Live-cell imaging, Flow Cytometry, IHC/ICC, Super-resolution microscopy – STED, High-content screening |
---|---|---|---|---|---|---|---|
MitoBrilliant™ Live 646 Cat. No. 7417 | Janelia Fluor® technology | 648/662 | Yes | Live-cell work only | No | Yes, but replacing media recommended | Live-cell imaging, Flow Cytometry, High-content screening |
MitoBrilliant™ Live 549 Cat. No. 7693 | Janelia Fluor® technology | 550/568 | Yes | Live-cell work only | No | Yes, but replacing media recommended | Live-cell imaging, Flow Cytometry, High-content screening |
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 416.95 |
储存 | Store at -20°C |
纯度 | ≥95% (HPLC) |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
DMSO | 20.85 | 50 |
以下数据基于产品分子量 416.95。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.5 mM | 4.8 mL | 23.98 mL | 47.97 mL |
2.5 mM | 0.96 mL | 4.8 mL | 9.59 mL |
5 mM | 0.48 mL | 2.4 mL | 4.8 mL |
25 mM | 0.1 mL | 0.48 mL | 0.96 mL |
参考文献是支持产品生物活性的出版物。
If you know of a relevant reference for MitoBrilliant™ Live 549, please let us know.
关键词: MitoBrilliant Live 549, MitoBrilliant Live 549 supplier, MitoBrilliant, orange, fluorescent, mitochondrial, marker, stain, mitotracker, mitochondria, live, cell, imaging, Flow, Cytometry, Mitobrilliant, 7693, Tocris Bioscience
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