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Mpro Tracer

Cat. No. 8126 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： SARS-CoV-2 main protease fluorescent ligand; TR-FRET acceptor
纯度： ≥95% (HPLC)
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生物活性 Scientific Data 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Mpro Tracer

Mpro Tracer is a high affinity (Kd = 40 nM) non-covalent SARS-CoV-2 main protease fluorescent ligand. Mpro Tracer can be used in fluorescent polarisation (FP) assay for inhibitor screening/characterisation. It can be used as a FRET acceptor in time-resolved fluorescence resonance energy-transfer (TR-FRET) experiments. Excitation and emission maxima (λ) are 492 nm and 518 nm.

Scientific Data

Inhibition Activity Fluorescence polarisation assay to determine the inhibitory activity of nirmatrelvir on purified SARS-CoV-2 Main protease protein View Larger

Fluorescence polarisation assay to determine the inhibitory activity of nirmatrelvir on purified SARS-CoV-2 Main protease protein (Mpro) Mpro Tracer and purified Mpro were used at a concentration of 1 μM. A dose response over a range of inhibitor concentrations were measured. Assays were performed in 100 μL wells and left for 1 hour at room temperature before FP measurements were taken. Curve was plotted and fit using graphpad to obtain an IC50 value of 0.74 μM under the assay conditions. Data provided by the Hilgenfeld lab, Lubeck University.

Binding affinity TR-FRET to determine binding affinity of Mpro Tracer View Larger

3CLpro-C10778AA-FITC tracer HTRF assay The Kd of Mpro tracer was measured by varying the tracer concentration against a range of recombinant His6-tagged Mpro concentrations using an HTRF (TR-FRET) assay. MAb Anti-6HIS-Tb GOLD was used as the FRET donor and Mpro Tracer as the FRET acceptor (abs/em = 492nm/518nm). HTRF ratio (520/495) was determined using as Envision plate reader, measurement were recorded after 90 minutes. The Kd of Mpro Tracer was determined to be 40 nM.

Optical Data for Mpro Tracer

Mpro Tracer Dye Spectra
Emission Color Green
λabs 492 nm
λem 518 nm

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for Mpro Tracer

分子量 909.41
储存 Store at -20°C
纯度 ≥95% (HPLC)

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Mpro Tracer

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 9.09 10

制备储备液 for Mpro Tracer

以下数据基于产品分子量 909.41。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 11 mL 54.98 mL 109.96 mL
0.5 mM 2.2 mL 11 mL 21.99 mL
1 mM 1.1 mL 5.5 mL 11 mL
5 mM 0.22 mL 1.1 mL 2.2 mL

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参考文献 for Mpro Tracer

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关键词: Mpro Tracer, Mpro Tracer supplier, SARS-Cov-2, cov, sars, Fluorescent, Fluorescence, imaging, polarization, FP, assays, TR-FRET, assay, ligand, protease, acceptor, FRET, coronavirus, antiviral, Other, Probes, Coronavirus, and, Assay, Reagents, 8126, Tocris Bioscience

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Fluorescent Dyes and Probes Research Product Guide

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