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说明： Fluorescent Cl- indicator; membrane permeable
化学名： 1-(2-Ethoxy-2-oxoethyl)-6-methoxyquinolin-1-ium bromide
纯度： ≥95% (HPLC)
生物活性 for MQAE
MQAE is a membrane-permeable intracellular chloride ion (Cl-) indicator. The fluorescence intensity of MQAE decreases proportionally as Cl- ions increase. Excitation/emission maxima (λ) are 355 nm and 460 nm respectively. Can be used in fluorescence microscopy and flow cytometry, suitable for use in vivo and in vitro.
Optical Data for MQAE
| λabs | 355 nm |
|---|---|
| λem | 460 nm |
| Extinction Coefficient (ε) | 4850 M-1cm-1 |
| Cell Permeable | Yes |
| pH Sensitivity | Insensitive |
| Application | Confocal imaging, Flow cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for MQAE
| 分子量 | 326.19 |
| 公式 | C14H16BrNO3 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 162558-52-3 |
| PubChem ID | 2762651 |
| InChI Key | DSLLHVISNOIYHR-UHFFFAOYSA-M |
| Smiles | O=C(C[N+]1=CC=CC2=CC(OC)=CC=C21)OCC.[Br-] |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for MQAE
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 3.26 | 10 | |
| water | 3.26 | 10 |
制备储备液 for MQAE
以下数据基于产品分子量 326.19。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 30.66 mL | 153.28 mL | 306.57 mL |
| 0.5 mM | 6.13 mL | 30.66 mL | 61.31 mL |
| 1 mM | 3.07 mL | 15.33 mL | 30.66 mL |
| 5 mM | 0.61 mL | 3.07 mL | 6.13 mL |
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产品说明书 for MQAE
参考文献 for MQAE
参考文献是支持产品生物活性的出版物。
Verkman et al (1989) Synthesis and characterization of improved chloride-sensitive fluorescent indicators for biological applications. Anal.Biochem. 178 355 PMID: 2751097
Wan et al (2019) An ATP-regulated ion transport nanosystem for homeostatic perturbation therapy and sensitizing photodynamic therapy by autophagy inhibition of tumors. ACS Cent.Sci. 5 327 PMID: 30834321
If you know of a relevant reference for MQAE, please let us know.
关键词: MQAE, MQAE supplier, ion, indicator, membrane, permeable, chloride, cl, fluorescent, fluorescence, miscroscopy, flow, cytometry, in, vivo, vitro, Fluorescent, Ion, Indicators, 7856, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals