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Submit ReviewNile Red
说明： Fluorogenic lipid membrane dye. Used for: intracellular lipid droplets and neutral lipids staining, suitable for live cell imaging. Application: fluorescence microscopy
别名： Nile blue oxazone
化学名： 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
纯度： ≥98% (HPLC)
生物活性 for Nile Red
Key information: Nile red is a fluorogenic lipid membrane dye.
Used for: staining of intracellular lipid droplets and neutral lipids. It is suitable for long-term monitoring of lipid dynamics in living cells.
Application: fluorescence microscopy.
Properties and Photophysical Data: Nile red fluoresces in the hydrophobic environment of lipid membranes. Non-fluorescent in polar environments (e.g. water). Excitation and emission maxima (λ) are 552 nm and 636 nm in methanol, respectively.
Optical Data for Nile Red
| Emission Color | Red |
|---|---|
| λabs | 552 nm |
| λem | 636 nm |
| Application | Fluorescence Microscopy, Monitoring of Lipid Dynamics in Living Cells |
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技术数据 for Nile Red
| 分子量 | 318.38 |
| 公式 | C20H18N2O2 |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 7385-67-3 |
| PubChem ID | 65182 |
| InChI Key | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| Smiles | CCN(C1=CC2=C(N=C3C(O2)=CC(C4=CC=CC=C34)=O)C=C1)CC |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Nile Red
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 3.18 | 10 |
制备储备液 for Nile Red
以下数据基于产品分子量 318.38。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 31.41 mL | 157.05 mL | 314.09 mL |
| 0.5 mM | 6.28 mL | 31.41 mL | 62.82 mL |
| 1 mM | 3.14 mL | 15.7 mL | 31.41 mL |
| 5 mM | 0.63 mL | 3.14 mL | 6.28 mL |
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产品说明书 for Nile Red
参考文献 for Nile Red
参考文献是支持产品生物活性的出版物。
Maes et al (2017) A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile red. Sci.Rep 16 44501 PMID: 28300146
Zhanghao et al (2020) High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. Nat.Commun. 11 5890 PMID: 33208737
Uribe-Etxebarria et al (2019) Wnt signaling reprograms metabolism in dental pulp stem cells. J.Cell Physiol. 234 13068 PMID: 30549037
If you know of a relevant reference for Nile Red, please let us know.
关键词: Nile Red, Nile Red supplier, fluorogenic, lipids, membranes, red, dye, Nile, blue, oxazone, Fluorescent, Lipid, Probes, and, Cell, Membrane, Stains, 7387, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals