Pepstatin A Janelia Fluor^® 526

Name: Pepstatin A Janelia Fluor ® 526
Brand: Tocris Bioscience
SKU: 7314
Rating: 4 (1 reviews)

Cat. No. 7314 1 条评论提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Pricing	Availability		Qty

说明： Fluorogenic green-emitting lysosome tracker and stain

别名： Pepstatin A JF526

化学名： 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((6S,9S,12S,13S,17S,20S,21S)-13,21-dihydroxy-12,20-diisobutyl-6,9-diisopropyl-2,17-dimethyl-4,7,10,15,18,23-hexaoxo-5,8,11,16,19,24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate

纯度： ≥90% (HPLC)

说明书

引用文献

生物活性 Scientific Data 技术数据溶解性计算器说明书参考文献

生物活性 for Pepstatin A Janelia Fluor^® 526

Pepstatin A Janelia Fluor^® 526 is a fluorogenic green-emitting lysosome tracker and stain. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor^® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

Scientific Data

Confocal Microscopy

Application of Pepstatin A Janelia Fluor<sup>®</sup> 526 in live U2OS cells.

View Larger

Application of Pepstatin A Janelia Fluor^® 526 in live U2OS cells. Live U2OS cells were stained with Pepstatin A Janelia Fluor^® 526 (2 µM) for 1 hour with Verapamil (10 µM) and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.

Confocal Microscopy

Application of Pepstatin A Janelia Fluor<sup>®</sup> 526 in fixed U2OS cells.

View Larger

Application of Pepstatin A Janelia Fluor^® 526 in fixed U2OS cells. Fixed U2OS cells were stained with Pepstatin A Janelia Fluor^® 526 (2 µM) for 1 hour in 0.1 M sodium acetate buffer pH 4.5. Cells were imaged on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Pepstatin A Janelia Fluor^® 526

Pepstatin A Janelia Fluor<sup>®</sup> 526 Dye Spectra

Emission Color	Yellow
λ_abs	530 nm
λ_em	549 nm
Extinction Coefficient (ε)	118,000 M^-1cm^-1
Quantum Yield (φ)	0.87
Application	Confocal Microscopy, STED, 3D-SIM, Lattice Light-sheet Microscopy
Alternative For	Lysotracker™- Lysotracker is a registered trademark of Thermo Fisher

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Pepstatin A Janelia Fluor^® 526

分子量	1442.53
公式	C₆₇H₉₁F₆N₉O₁₂.CF₃CO₂H
储存	Store at -20°C
纯度	≥90% (HPLC)
CAS Number	2410614-07-0
InChI Key	JONSFJPZCPEZFP-WKVYEHBGSA-N
Smiles	OC(C(F)(F)F)=O.CC(C[C@@H]([C@H](CC(NCCCCCCNC(C1=CC(C2=C3C=C(/C(C=C3OC4=C2C=C(C(N5CC(F)(C5)F)=C4)F)=[N+]6CC(F)(C\6)F)F)=C(C([O-])=O)C=C1)=O)=O)O)NC([C@@H](NC(C[C@@H]([C@@H](NC([C@H](C(C)C)NC([C@H](C(C)C)NC(CC(C)C)=O)=O)=O)CC(C)C)O)=O)C)=O)C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Pepstatin A Janelia Fluor^® 526

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	14.43	10

制备储备液 for Pepstatin A Janelia Fluor^® 526

以下数据基于产品分子量 1442.53。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.1 mM	6.93 mL	34.66 mL	69.32 mL
0.5 mM	1.39 mL	6.93 mL	13.86 mL
1 mM	0.69 mL	3.47 mL	6.93 mL
5 mM	0.14 mL	0.69 mL	1.39 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt* g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1

Volume 1

Concentration 2

Volume 2

产品说明书 for Pepstatin A Janelia Fluor^® 526

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Pepstatin A Janelia Fluor^® 526

参考文献是支持产品生物活性的出版物。

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

Chen et al (2000) Probing the cathepsin D using a BODIPY FL-pepstatin A: applications in fluorescence polarization and microscopy. J.Biochem.Biophys.Methods 42 137 PMID: 10737220

If you know of a relevant reference for Pepstatin A Janelia Fluor^® 526, please let us know.

按标靶查看相关产品

关键词: Pepstatin A Janelia Fluor 526, Pepstatin A Janelia Fluor 526 supplier, Fluorogenic, green, emitting, lysosomes, lysosomal, probes, stains, dyes, tracker, staining, Pepstatin, A, JF526, Fluorescent, Lysosome, Probes, 7314, Tocris Bioscience

篇 Pepstatin A Janelia Fluor^® 526 的引用文献

引用文献是使用了 Tocris 产品的出版物。

目前没有 Pepstatin A Janelia Fluor^® 526 的引用文献。您是否知道使用了 Tocris Pepstatin A Janelia Fluor^® 526 的优秀论文？请告知我们.