Phalloidin-FITC

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说明: Green-fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
化学名: 7-[(4R)-N5-[[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)amino]thioxomethyl]-4-hydroxy-4-methyl-L-ornithine]phalloidin
纯度: ≥90% (HPLC)
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引用文献
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生物活性 for Phalloidin-FITC

Key information: Phalloidin-FITC is a green-fluorescent cytoskeleton stain. It binds and labels specifically F-actin.

Used for: Phalloidin-FITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: fluorescent microscopy, immunofluorescence (IHC, ICC).

Properties and Photophysical Data: Phalloidin-FITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Fluorescein isothiocyanate (FITC; Cat. No. 5440) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-FITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 496 nm and 516 nm, respectively.

We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO. Please refer to the protocol for further information on using this product.

Scientific Data

Fluorescence Imaging Applications in HeLa Cells for Phalloidin-FITC View Larger

Applications in HeLa Cells for Phalloidin-FITC. Application of Phalloidin-FITC: HeLa cells were labeled with 150 nM Phalloidin-FITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).

Optical Data for Phalloidin-FITC

λabs 496 nm
λem 516 nm
Application Fluorescent Microscopy, Immunofluorescence (IHC, ICC).

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for Phalloidin-FITC

分子量 1177.26
公式 C56H60N10O15S2
储存 Store at -20°C
纯度 ≥90% (HPLC)
CAS Number 915026-99-2
PubChem ID 131676694
InChI Key CJEWZWZNRFVJSM-PNHWZQMUSA-N
Smiles C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C(O)=O)=C(C=C2)C2=C3C=CC(=O)C=C3OC3=C2C=CC(O)=C3)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

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关键词: Phalloidin-FITC, Phalloidin-FITC supplier, green, fluorescent, actin, stain, phalloidin, fluorescein, filament, probe, Organelle, and, Cell, Structure, Stains, Fluorescent, Actin, Probes, 5782, Tocris Bioscience

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Phalloidin-FITC 实验方案

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