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Submit ReviewPropidium iodide
说明： Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, nuclear counterstaining, viability staining. Application: flow cytometry, confocal microscopy, fluorescence microscopy
别名： PI
化学名： 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
纯度： ≥95% (HPLC)
生物活性 for Propidium iodide
Key information: Propidium iodide (PI) is a widely used red-fluorescent DNA stain. It is membrane impermeant to live cells and so PI staining differentiates live and dead cells. PI can be used to stain nucleic acids in dead cells across different cell types, including mammalian cells, bacteria, and yeast.
Application: flow cytometry, confocal microscopy, fluorescence microscopy.
Used for: apoptosis detection by PI staining, nuclear counterstaining in multicolor fluorescent assays, cell death measuring in a mixed live-cell population by PI staining and flow cytometry.
Properties and Photophysical Data: Propidium iodide fluorescence increases by approximately 20- to 30-fold upon binding to DNA or RNA (through intercalation). When unbound, excitation and emission maxima (λ) are 493 nm and 636 nm, respectively, when bound to DNA, excitation and emission maxima (λ) are 535 nm and 617 nm, respectively.
Optical Data for Propidium iodide
| Emission Color | Orange |
|---|---|
| λabs | 535 nm |
| λem | 617 nm |
| Application | Flow Cytometry, Fluorescence Microscopy, Confocal Microscopy |
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技术数据 for Propidium iodide
| 分子量 | 668.39 |
| 公式 | C27H34I2N4 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 25535-16-4 |
| PubChem ID | 104981 |
| InChI Key | XJMOSONTPMZWPB-UHFFFAOYSA-M |
| Smiles | NC4=CC1=C(C=C4)C3=C(C=C(N)C=C3)[N+](CCC[N+](CC)(CC)C)=C1C2=CC=CC=C2.[I-].[I-] |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Propidium iodide
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| water | 3.34 | 5 超声处理 |
制备储备液 for Propidium iodide
以下数据基于产品分子量 668.39。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.05 mM | 29.92 mL | 149.61 mL | 299.23 mL |
| 0.25 mM | 5.98 mL | 29.92 mL | 59.85 mL |
| 0.5 mM | 2.99 mL | 14.96 mL | 29.92 mL |
| 2.5 mM | 0.6 mL | 2.99 mL | 5.98 mL |
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产品说明书 for Propidium iodide
参考文献 for Propidium iodide
参考文献是支持产品生物活性的出版物。
Jeong et al (2013) SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma. J.Biol.Chem. 289 4135 PMID: 24368766
Sonnemann et al (2014) p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors. Br.J.Cancer 110 656 PMID: 24281001
Yun et al (2013) Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J.Biol.Chem. 289 1491 PMID: 24297180
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关键词: Propidium iodide, Propidium iodide supplier, red, fluorescent, DNA, stain, dye, cell, death, PI, Fluorescent, Stains, Cell, Viability, and, Dyes, Flow, Cytometry, 5135, Tocris Bioscience
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Propidium iodide 的评论
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MCF7 Spheroids cell death.
By
Caterina Suelzu on 11/26/2024
分析类型： In Vitro
种属： Human
细胞系/组织： MCF7
MCF7 cells spheroids were stained with 3 uM Propidium Iodide for 1.5h
Staining of HCT116 spheroids.
By
Gabriele Strusi on 11/26/2024
分析类型： In Vitro
种属： Human
细胞系/组织： HCT116
Staining of HCT 116 spheroids using a 3 uM concentration
Good Product.
By
Rohit Sharma on 08/17/2020
分析类型： In Vitro
种属： Human
细胞系/组织： Jurkat Cells
PI was used in the cell cycle analysis of Jurkat cells. The cell pellet of the treated cells was resuspended in 200 μL propidium iodide (PI) solution containing 50 μg/mL PI, 0.1 mg/mL RNase A, and 0.05% Triton X-100 (v/v) (prepared in 1X PBS buffer). The cells were incubated for 30 min. in the dark at room temperature and by agitating every 10 mins. After incubation, the samples were analyzed using the flow cytometer.
Propidium dye for intracellular fluorescent dye..
By
Anonymous on 03/13/2018
分析类型： Ex Vivo
种属： Mouse
Propidium Iodide was used as an intracellular fluorescent dye. Picture is of the glass electrode with a 5mM solution. 250uM was also tried but had poor visibility.
5mM is great for visualization with an mcherry filter. It goes into water well.
该领域的文献
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals