主页 / Product Type / 荧光成像 / Fluorescent Probes and Stains / Fluorescent RNA Probes / RNA Imaging Probe 1c

RNA Imaging Probe 1c

Cat. No. 8813 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Fluorogenic RNA imaging probe
化学名： 3-[(E)-2-(1-Methylpyridin-1-ium-4-yl)ethenyl]indolizine iodide
纯度： ≥98% (HPLC)
说明书
引用文献
评论
文献 (1)
生物活性 Scientific Data 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for RNA Imaging Probe 1c

RNA Imaging Probe 1c is a fluorogenic RNA imaging probe. RNA Imaging Probe 1c displays high membrane permeability, strong fluorogenic responses upon binding RNA, compatibility with fluorescence lifetime imaging microscopy (FLIM), low cytotoxicity, and excellent photostability. Excitation and emission maxima (λ) are 556 nm and 608 nm, respectively; quantum yield = 0.49; extinction coefficient = 27,500 M-1cm-1. Suitable for live cell imaging.

Scientific Data

Confocal Microscopy Live HeLa cells labeled with RNA Imaging Probe 1c View Larger

Application of RNA Imaging Probe 1c on live HeLa cells. Confocal fluorescence images of live Hela cells imaged with 20 μM methyl pyridinium indole (MPI) (green) ( lambda ex = 470 nm, lambda em = 525-555 nm) and 20 μM RNA Imaging Probe 1c (red) ( lambda ex = 550 nm, lambda em= 580-620 nm) for 30 min. Scale bar: 10 μm.

Confocal Microscopy DAPI counterstaining alongside RNA Imaging Probe 1c on HeLa cells View Larger

Application of RNA Imaging Probe 1c on PFA-fixed HeLa cells. Confocal fluorescence images of PFA-fixed HeLa cells incubated with 20 μM RNA Imaging Probe 1c (red) and 1 μg/mL Hoechst 33342 (blue) for 30 min.

Confocal Microscopy HeLa cells stained with RNA Imaging Probe 1c View Larger

Application of RNA Imaging Probe 1c on HeLa cells. Zoomed-in confocal fluorescence image of HeLa cells stained with 20 μM RNA Imaging Probe 1c for 30 min. Image shows the sub-nucleolar components fibrillar center (FC) (indicated by the white arrowhead), dense fibrillar component (DFC), and outer granular component (GC) (indicated by the white arrows). Scale bar: 3 μm.

Fluorescence Imaging Yeast RNA and spermine labeled with RNA Imaging Probe 1c View Larger

Yeast RNA and spermine labeled with RNA Imaging Probe 1c. Torula yeast RNA and spermine were incubated with RNA Imaging Probe 1c (10 μM). FLIM images show the formation of spherical coacervate droplets upon mixing the RNA and spermine. Image is enlarged by 1× (on the left) or by 8× (on the right). Scale bar: 10 μm.

许可信息

Sold under license from the University of Southern California.

Optical Data for RNA Imaging Probe 1c

λabs 556 nm
λem 608 nm
Extinction Coefficient (ε) 27500 M-1cm-1
Quantum Yield (φ) 0.49
Cell Permeable Yes

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for RNA Imaging Probe 1c

分子量 362.21
公式 C16H15IN2
储存 Store at -20°C
纯度 ≥98% (HPLC)
PubChem ID 171378441
InChI Key ODMXTRDMWRHIDS-UHFFFAOYSA-M
Smiles C[N+]1=CC=C(/C=C/C2=CC=C3N2C=CC=C3)C=C1.[I-]

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for RNA Imaging Probe 1c

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 7.24 20

制备储备液 for RNA Imaging Probe 1c

以下数据基于产品分子量 362.21。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.2 mM 13.8 mL 69.02 mL 138.04 mL
1 mM 2.76 mL 13.8 mL 27.61 mL
2 mM 1.38 mL 6.9 mL 13.8 mL
10 mM 0.28 mL 1.38 mL 2.76 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

产品说明书 for RNA Imaging Probe 1c

分析证书/产品说明书
化学品安全技术说明书

参考文献 for RNA Imaging Probe 1c

参考文献是支持产品生物活性的出版物。

Kim et al (2023) Development of highly fluorogenic styrene probes for visualizing RNA in live cells. ACS Chem.Biol. 18 1523 PMID: 37200527

If you know of a relevant reference for RNA Imaging Probe 1c, please let us know.

按标靶查看相关产品

关键词: RNA Imaging Probe 1c, RNA Imaging Probe 1c supplier, RNA, imaging, probe-1c, fluorescent, fluorophore, probe, probes, live, confocal, microscopy, membrane, permeable, fluorogenic, FLIM, photostability, Fluorescent, Probes, 8813, Tocris Bioscience

篇 RNA Imaging Probe 1c 的引用文献

引用文献是使用了 Tocris 产品的出版物。

目前没有 RNA Imaging Probe 1c 的引用文献。 您是否知道使用了 Tocris RNA Imaging Probe 1c 的优秀论文？ 请告知我们.

RNA Imaging Probe 1c 的评论

目前没有该产品的评论。 Be the first to review RNA Imaging Probe 1c and earn rewards!

Have you used RNA Imaging Probe 1c?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals