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SAG dihydrochloride

Cat. No. 6390 3 篇引用文献 1 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Dihydrochloride salt of SAG; Smo receptor agonist
化学名： 3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide dihydrochloride
纯度： ≥98% (HPLC)
说明书
引用文献 (3)
评论 (1)
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for SAG dihydrochloride

SAG dihydrochloride is a water-soluble dihydrochloride salt of SAG (Cat. No. 4366). SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons. The compound can be used in protocols for the chemical reprogramming of somatic cells to iPSCs.

化合物库 for SAG dihydrochloride

SAG dihydrochloride is also offered as part of the Tocriscreen 2.0 Max. 了解 Tocris 化合物库的更多信息。

技术数据 for SAG dihydrochloride

分子量 562.98
公式 C28H28ClN3OS.2HCl
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 2702366-44-5
PubChem ID 71433770
InChI Key SWBYSYANHQTINY-CZBZHNTRSA-N
Smiles ClC1=C(C(N([C@H]2CC[C@H](NC)CC2)CC3=CC=CC(C4=CC=NC=C4)=C3)=O)SC5=CC=CC=C51.Cl.Cl

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for SAG dihydrochloride

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
water 56.3 100
DMSO 56.3 100

制备储备液 for SAG dihydrochloride

以下数据基于产品分子量 562.98。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 1.78 mL 8.88 mL 17.76 mL
5 mM 0.36 mL 1.78 mL 3.55 mL
10 mM 0.18 mL 0.89 mL 1.78 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

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产品说明书 for SAG dihydrochloride

分析证书/产品说明书
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化学品安全技术说明书

参考文献 for SAG dihydrochloride

参考文献是支持产品生物活性的出版物。

Guan et al (2022) Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 605 325 PMID: 35418683

If you know of a relevant reference for SAG dihydrochloride, please let us know.

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关键词: SAG dihydrochloride, SAG dihydrochloride supplier, Potent, Smoothened, Smo, receptors, agonists, activates, Hedgehog, signaling, pathway, activator, enhancers, neuronal, differentiation, iPSCs, induced, pluripotent, stem, cells, dopaminergic, neurons, neurones, 912545-86-9, reprogramming, Receptors, Neural, Stem, Cells, Cell, Reprogramming, 6390, Tocris Bioscience

3 篇 SAG dihydrochloride 的引用文献

引用文献是使用了 Tocris 产品的出版物。 SAG dihydrochloride 的部分引用包括：

Lucia et al (2020) HDL inhibits endoplasmic reticulum stress-induced apoptosis of pancreatic β-cells in vitro by activation of Smoothened. J Lipid Res 61 492-504 PMID: 31907205

Madeline A et al (2021) Population-scale single-cell RNA-seq profiling across dopaminergic neuron differentiation. Nat Genet 53 304-312 PMID: 33664506

Joaquin M et al (2018) Trisomy 21 Represses Cilia Formation and Function. Dev Cell 46 641-650.e6 PMID: 30100262

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SAG dihydrochloride 的评论

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MUCH better than the others!!.
By Anonymous on 08/28/2020
分析类型： In Vitro
种属： Human
细胞系/组织： stem cells

This SAG performs the BEST for stem cell differentiation to neuronal lineages. Other brands do not work. We use it at 1uM after dual SMAD inhibition.

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products