sCy5DA

Pricing Availability   Qty
说明: FDAA for super-resolution microscopy of bacteria
化学名: (R)-2-Ammonio-3-(6-((E)-3,3-dimethyl-5-sulfo-2-((2E,4E)-5-(1,3,3-trimethyl-5-sulfonato-3H-indol-1-ium-2-yl)penta-2,4-dien-1-ylidene)indolin-1-yl)hexanamido)propanoate
纯度: ≥95% (HPLC)
说明书
引用文献
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文献 (1)

生物活性 for sCy5DA

sCy5DA is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans in live bacteria. It is efficiently incorporated into the peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into gram-negative C. crescentus. sCy5DA labelled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/665 nm.

Scientific Data

Fluorescence Imaging Vibrio cholerae cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Vibrio cholerae cells labeled with 500 μM sCy5DA

Fluorescence Imaging Bacillus subtilis cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA

Fluorescence Imaging Mycobacterium smegmatis cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Mycobacterium smegmatis cells labeled with 100 μM sCy5DA

Fluorescence Imaging Staphylococcus aureus cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Staphylococcus aureus labeled with 100 μM sCy5DA

Optical Data for sCy5DA

sCy5DA Dye Spectra
Emission Color Red
λabs 646 nm
λem 665 nm
Extinction Coefficient (ε) 197,240 M-1cm-1
Cell Permeable Yes
Application STORM

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for sCy5DA

分子量 728.88
公式 C35H44N4O9S2
储存 Store at -20°C
纯度 ≥95% (HPLC)
InChI Key ZSTSSXALPFZNBO-HHHXNRCGSA-N
Smiles CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(NC[C@H](C([O-])=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for sCy5DA

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 7.29 10

制备储备液 for sCy5DA

以下数据基于产品分子量 728.88。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 13.72 mL 68.6 mL 137.2 mL
0.5 mM 2.74 mL 13.72 mL 27.44 mL
1 mM 1.37 mL 6.86 mL 13.72 mL
5 mM 0.27 mL 1.37 mL 2.74 mL

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参考文献 for sCy5DA

参考文献是支持产品生物活性的出版物。

Zhang et al (2022) Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 17 2418 PMID: 35994360


If you know of a relevant reference for sCy5DA, please let us know.

关键词: sCy5DA, sCy5DA supplier, fluorescent, D-amino, acid, FDAA, peptidoglycans, labeling, labels, labelling, amino, acids, probes, for, imaging, bacteria, SMLM, PALM, STORM, Fluorescent, Probes, Imaging, Bacteria, 7834, Tocris Bioscience

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

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