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说明： Fluorogen for SiRA light-up aptamer. Application: confocal microscopy, SRM (including STED)
化学名： (5-((2-(2-(2-Aminoethoxy)ethoxy)ethyl)carbamoyl)-2-(7-(dimethylamino)-3-(dimethyliminio)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate)
纯度： ≥98% (HPLC)
生物活性 for SiRA 2
Key information: SiRA 2 (silicon rhodamine 2) is a near-infrared (NIR) fluorogen for SiRA light-up aptamer. SiRA 2 enables imaging of single RNA in living cells.
Application: confocal microscopy, super resolution microscopy (SRM) techniques, including super-resolution stimulated emission depletion (STED). Cell permeable.
Properties and Photophysical Data: SiRA 2 is extremely bright, photostable and allows the visualization of mRNA expression in bacteria in no-wash, quencher-free, live-cell imaging experiments. Excitation and emission maxima (λ) are 649 nM and 662 nM, respectively; quantum yield = 0.98; extinction coefficient = 86,000 M-1cm-1; KD = 403 nM.
Reconstitute in 200 μL for a 0.5 mM stock solution. A typical working concentration for this product is 500 nM, see references provided for more details.
We recommend using 5mM Mg2+ (MgCl2 or MgSO4) in all buffers/media for maximum fluorescence intensity.
许可信息
Sold with kind permission of Prof. Andres Jäschke
Optical Data for SiRA 2
| Emission Color | Far Red |
|---|---|
| λabs | 649 nm |
| λem | 662 nm |
| Extinction Coefficient (ε) | 86000 M-1cm-1 |
| Quantum Yield (φ) | 0.98 |
| Closest Laser line | 640 nm |
| Cell Permeable | Yes |
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技术数据 for SiRA 2
| 分子量 | 602.81 |
| 公式 | C33H42N4O5Si |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 2365417-91-8 |
| PubChem ID | 168440271 |
| InChI Key | VZACMAUVTBSQSR-UHFFFAOYSA-N |
| Smiles | CN(C1=CC=C2C([Si](C)(C3=C/C(C=CC3=C2C4=C(C([O-])=O)C=C(C(NCCOCCOCCN)=O)C=C4)=[N+](C)\C)C)=C1)C |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for SiRA 2
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 60.28 | 100 | |
| water | 30.14 | 50 |
制备储备液 for SiRA 2
以下数据基于产品分子量 602.81。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.66 mL | 8.29 mL | 16.59 mL |
| 5 mM | 0.33 mL | 1.66 mL | 3.32 mL |
| 10 mM | 0.17 mL | 0.83 mL | 1.66 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.33 mL |
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产品说明书 for SiRA 2
参考文献 for SiRA 2
参考文献是支持产品生物活性的出版物。
Wirth et al (2019) SiRA: A silicon rhodamine-binding aptamer for live-cell super-resolution RNA imaging. J.Am.Chem.Soc. 141 7562 PMID: 30986047
If you know of a relevant reference for SiRA 2, please let us know.
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关键词: SiRA 2, SiRA 2 supplier, SiRA2, fluorescence, fluorophores, single, RNA, imaging, fluorescent, aptamers, aptamer-based, silicon, rhodamine, NIR, near-infrared, live, cell, STED, SRM, Aptamer-based, Imaging, Fluorescent, Probes, 7544, Tocris Bioscience
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该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals