TBI

Cat. No. 7660 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Fluorogenic ligand for Broccoli RNA aptamer

化学名： (Z)-3-((1H-Benzo[d]imidazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-thioxoimidazolidin-4-one

纯度： ≥98% (HPLC)

说明书

引用文献

文献 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for TBI

TBI is a DFHBI derivative for imaging of single Broccoli-tagged mRNA in cells. Excitation and emission maxima (λ) when bound to Broccoli are 485 and 527 nm, respectively. TBI shows higher affinity for Broccoli aptamers compared with DFHBI-1T (Cat. No. 5610) (K_D values are 71 and 305 nM, respectively). Extinction coefficient = 35,100 M^-1 cm^-1.

许可信息

Sold under license from Lucerna

Optical Data for TBI

Emission Color	Green
λ_abs	485 nm
λ_em	527 nm
Extinction Coefficient (ε)	35100 M^-1cm^-1
Quantum Yield (φ)	0.64
Application	Live-cell Imaging, Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for TBI

分子量	386.38
公式	C₁₈H₁₂F₂N₄O₂S
储存	Store at -20°C
纯度	≥98% (HPLC)
CAS Number	2863639-29-4
InChI Key	FFVXYOZVQLWDIF-NSIKDUERSA-N
Smiles	FC1=C(C(F)=CC(/C=C2NC(N(C\2=O)CC3=C4N=CNC4=CC=C3)=S)=C1)O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for TBI

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	7.73	20

制备储备液 for TBI

以下数据基于产品分子量 386.38。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.2 mM	12.94 mL	64.7 mL	129.41 mL
1 mM	2.59 mL	12.94 mL	25.88 mL
2 mM	1.29 mL	6.47 mL	12.94 mL
10 mM	0.26 mL	1.29 mL	2.59 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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产品说明书 for TBI

分析证书/产品说明书

化学品安全技术说明书

参考文献 for TBI

参考文献是支持产品生物活性的出版物。

Li et al (2021) Engineering fluorophore recycling in a fluorogenic RNA aptamer. Angew.Chem.Int.Ed.Engl. 60 24153 PMID: 34490956

If you know of a relevant reference for TBI, please let us know.

按标靶查看相关产品

关键词: TBI, TBI supplier, fluorescence, fluorophores, fluorophore, single, RNA, imaging, fluorescent, protein, Broccoli, aptamers, Aptamer-based, Imaging, 7660, Tocris Bioscience

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该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

Fluorescent Dyes, including dyes for flow cytometry
Fluorescent Probes and Stains, including our new MitoBrilliant^TM mitochondria stains
Tissue Clearing Kits and Reagents
Aptamer-based RNA Imaging Reagents
Fluorescent Probes for Imaging Bacteria
TSA Vivid^TM Fluorophore Kits
TSA Reagents for Enhancing IHC, ICC & FISH Signals

TBI