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Submit ReviewKey information: Thioflavin T is a cell-permeable fluorescent amyloid stain.
Used for: staining of insoluble senile plaques of Aβ in brain tissues, monitoring the kinetics of in vitro polyglutamine amyloid formation of tNhtt-42Q aggregates in a Huntington's disease cell model. Thioflavin T is used to confirm formation of β sheet structure from mutant huntingtin exon-1 aggregates (Ex1Q48) in vitro.
Application: fluorescence microscopy.
Properties and Photophysical Data: Thioflavin T is a cationic benzothiazole. It increases in fluorescence upon strong binding (Ki = 580 nM) to the stacked β sheets of amyloid fibrils. Excitation and emission maxima (λ) are 440 nm and 485 nm, respectively; quantum yield = 0.43; extinction coefficient = 31600 M-1cm-1.
This product is supplied with a high degree of hydration and some residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Emission Color | Blue |
---|---|
λabs | 440 nm |
λem | 485 nm |
Extinction Coefficient (ε) | 31600 M-1cm-1 |
Quantum Yield (φ) | 0.43 |
Cell Permeable | Yes |
Application | Amyloid Stain |
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 318.86 |
公式 | C17H19ClN2S |
储存 | Store at -20°C |
纯度 | ≥95% (HPLC) |
CAS Number | 2390-54-7 |
PubChem ID | 16953 |
InChI Key | JADVWWSKYZXRGX-UHFFFAOYSA-M |
Smiles | CC1=CC2=C([N+](C)=C(C3=CC=C(N(C)C)C=C3)S2)C=C1.[Cl-] |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
DMSO | 1.59 | 5 | |
water | 3.19 | 10 |
以下数据基于产品分子量 318.86。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.1 mM | 31.36 mL | 156.81 mL | 313.62 mL |
0.5 mM | 6.27 mL | 31.36 mL | 62.72 mL |
1 mM | 3.14 mL | 15.68 mL | 31.36 mL |
5 mM | 0.63 mL | 3.14 mL | 6.27 mL |
参考文献是支持产品生物活性的出版物。
Doi et al (2008) RNA-binding protein TLS is a major nuclear aggregate-interacting protein in huntingtin exon 1 with expanded polyglutamine-expressing cells. J.Biol.Chem. 283 6489 PMID: 18167354
Lee et al (2019) Fluorescence chemicals to detect insoluble and soluble amyloid-β aggregates. ACS Chem.Neurosci. 10 2647 PMID: 31009195
Schindler (2021) Small, seeding-competent huntingtin fibrils are prominent aggregate species in brains of zQ175 Huntington's disease knock-in mice. Front.Neurosci. 15 682172 PMID: 34239412
If you know of a relevant reference for Thioflavin T, please let us know.
关键词: Thioflavin T, Thioflavin T supplier, thioflavint, cell-permeable, fluorescent, amyloid, stain, cationic, benzothiazole, dye, stacked, beta, sheets, fibrils, Fluorescent, Amyloid, B, Probes, 7122, Tocris Bioscience
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