Tissue Clearing Pro-Organoid

Cat. No. 7390 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： 3D cell culture clearing reagent kit

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生物活性 Scientific Data 技术数据说明书参考文献

生物活性 for Tissue Clearing Pro-Organoid

Tissue Clearing Pro-Organoid is a rapid, easy-to-use, reversible and non-destructive kit, which allows effective tissue clearing and staining of organoids, 3D cell cultures and microtissues. Tissue Clearing Pro-Organoid is a solvent based tissue clearing technique, comparable to BABB and DISCO. Tissue Clearing Pro-Organoid does not cause significant shrinkage or tissue damage and does not use as harsh a solvent as other solvent based techniques.

This organoid tissue clearing kit allows 3D cell cultures up to 500 μm thick to be cleared in minutes, and is compatible with fluorescent protein, immunolabeling and small molecule staining techniques, as well as being suitable for use with all standard dyes and buffers. Following 3D imaging, Tissue Clearing Pro-Organoid can be reversed for follow-up 2D histology staining. The kit comes with a getting started guide and a more comprehensive tissue clearing guidebook, providing detailed protocols. Tissue Clearing Pro-Organoid kit contains 30 mL of Tissue Clearing Pro-Organoid clearing reagent plus the 4 buffer solutions required to process 3D cell cultures for immunolabeling and is enough to process three 96-well plates of material.

Please read the Tissue Clearing Pro-Organoid Guidebook for more information and detailed protocols for using this kit.

Scientific Data

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Tissue Clearing Pro-Organoid Applications. Tissue Clearing Pro-Organoid Kit (Catalog # 7390) was applied to open liver spheroids, allowing visualization of stained structures: Left DNA, MRPII and MDRI; Middle DNA, CD68, Albumin and Vimentin; Right DNA, panCK and CD31. A wide range of IHC validated antibodies are available from R&D Systems and Novus Biologicals; optimization of antibody incubation conditions for use with the Tissue Clearing Pro-Organoid technique is recommended.

技术数据 for Tissue Clearing Pro-Organoid

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上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for Tissue Clearing Pro-Organoid

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Tissue Clearing Pro-Organoid

参考文献是支持产品生物活性的出版物。

Buono et al (2020) Human cardiac organoids for modeling genetic cardiomyopathy. Cells 9 1733 PMID: 32698471

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