Tricyclic cytosine tC

Cat. No. 7814 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Fluorescent tricyclic cytosine analog for RNA imaging

化学名： 3-β-D-Ribofuranosyl-1H-pyrimido[5,4-b][1,4]benzothiazin-2(3H)-one

纯度： ≥95% (HPLC)

文献 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for Tricyclic cytosine tC

Tricyclic cytosine tC is a fluorescent cytosine analog for imaging RNA in living cells. Enables the investigation of RNA synthesis, degradation, and trafficking at single-cell resolution when combined with confocal imaging. Excitation/emission maxima (λ) = 375/505 nm; extinction coefficient = 4000 M^-1 cm^-1; quantum yield = 0.2.

Optical Data for Tricyclic cytosine tC

λ_abs	375 nm
λ_em	505 nm
Extinction Coefficient (ε)	4000 M^-1cm^-1
Quantum Yield (φ)	0.2
Application	Confocal Imaging

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技术数据 for Tricyclic cytosine tC

分子量	349.36
公式	C₁₅H₁₅N₃O₅S
储存	Store at -20°C
纯度	≥95% (HPLC)
CAS Number	1174063-74-1
InChI Key	SRXXFIYNNTZZBB-LHNIVKCTSA-N
Smiles	O[C@H]1[C@H](N2C(N=C3C(SC4=CC=CC=C4N3)=C2)=O)O[C@@H]([C@H]1O)CO

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Tricyclic cytosine tC

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	34.94	100

制备储备液 for Tricyclic cytosine tC

以下数据基于产品分子量 349.36。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	2.86 mL	14.31 mL	28.62 mL
5 mM	0.57 mL	2.86 mL	5.72 mL
10 mM	0.29 mL	1.43 mL	2.86 mL
50 mM	0.06 mL	0.29 mL	0.57 mL

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产品说明书 for Tricyclic cytosine tC

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Tricyclic cytosine tC

参考文献是支持产品生物活性的出版物。

Wang et al (2022) Live-cell RNA imaging with metabolically incorporated fluorescent nucleosides. J.Am.Chem.Soc. 144 14647 PMID: 35930766

Stengel et al (2010) Incorporation of the fluorescent ribonucleotide analogue tCTP by T7 RNA polymerase. Anal.Chem. 82 1082 PMID: 20067253

Sandin et al (2005) Fluorescent properties of DNA base analogue tC upon incorporation into DNA--negligible influence of neighbouring bases on fluorescence quantum yield. Nucleic Acids Res. 33 5019 PMID: 16147985

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