Home / Pharmacology / Transporters / Ion Pumps/Transporters / Na+/Ca2+ Exchanger / Na+/Ca2+ Exchanger Inhibitors / Benzamil

Benzamil

Cat. No. 3380 5 Citations Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: NCX inhibitor, Deg/ENaC channel blocker; amiloride (Cat. No. 0890) derivative
Chemical Name: N-(Benzylamidino)-3,5-diamino-6-chloropyrazinecarboxamide hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (5)
Reviews
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Benzamil

Benzamil is a Na+/Ca2+ exhanger (NCX) inhibitor (IC50 ~ 100 nM); TRPP3 channel blocker (IC50 = 1.1 μM for inhibition of Ca2+-activated TRPP3 channel activity). Also non-selective Deg/ENaC family blocker; reduces mechanosensitivity of colonic afferents. More potent derivative of amiloride (Cat. No. 0890).

Compound Libraries for Benzamil

Benzamil is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Benzamil

M. Wt 356.21
Formula C13H14ClN7O.HCl
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 161804-20-2
PubChem ID 5702295
InChI Key ZNWMRWWNJBXNKJ-UHFFFAOYSA-N
Smiles NC1=C(Cl)N=C(C(NC(NCC2=CC=CC=C2)=N)=O)C(N)=N1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Benzamil

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 35.62 100
water 3.56 10

Preparing Stock Solutions for Benzamil

The following data is based on the product molecular weight 356.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.81 mL 14.04 mL 28.07 mL
5 mM 0.56 mL 2.81 mL 5.61 mL
10 mM 0.28 mL 1.4 mL 2.81 mL
50 mM 0.06 mL 0.28 mL 0.56 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets for Benzamil

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References for Benzamil

References are publications that support the biological activity of the product.

Dai et al (2007) Inhibition of TRPP3 channel by amil. and analogs. Mol.Pharmacol. 72 1576 PMID: 17804601

Fischer et al (2002) Characterization of a Na+-Ca2+ exchanger in podocytes. Nephrol.Dial.Transplant. 17 1742 PMID: 12270979

Page et al (2007) Acid sensing ion channels 2 and 3 are required for inhibition of visceral nociceptors by benzamil. Pain 133 150 PMID: 17467171

If you know of a relevant reference for Benzamil, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Na+/Ca2+ Exchanger Inhibitors

Keywords: Benzamil, Benzamil supplier, non-selective, Deg, ENaC, family, NCX, sodium, calcium, Na+/Ca2+, exchanger, inhibitors, inhibits, amiloride, derivative, TRPP3, channel, blocker, Exchanger, Epithelial, Sodium, Channels, TRPP, 3380, Tocris Bioscience

5 Citations for Benzamil

Citations are publications that use Tocris products. Selected citations for Benzamil include:

Mizuno et al (2019) Development of Orthogonal Linear Separation Analysis (OLSA) to Decompose Drug Effects into Basic Components. Sci Rep 9 1824 PMID: 30755704

Panja et al (2019) Paraneoplastic cerebellar degeneration: Yo antibody alters mitochondrial calcium buffering capacity. Neuropathol Appl Neurobiol 45 141 PMID: 29679372

Agarwal et al (2017) Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes. Neuron 93 587 PMID: 28132831

Zou et al (2018) Knockout of mitochondrial voltage-dependent anion channel type 3 increases reactive oxygen species (ROS) levels and alters renal sodium transport. J Biol Chem 293 1666 PMID: 29180450

Do you know of a great paper that uses Benzamil from Tocris? Please let us know.

Reviews for Benzamil

There are currently no reviews for this product. Be the first to review Benzamil and earn rewards!

Have you used Benzamil?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review