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Submit ReviewSulforhodamine 101
Description: Red fluorescent dye; selective astrocyte marker
Chemical Name: 9-(2,4-Disulfophenyl)-2,3,6,7,12,13,16,17-octahydro-1H,5H,11H,15H-xantheno[2,3,4-ij:5,6,7-i'j']diquinolizin-18-ium inner salt
Purity: ≥95% (HPLC)
Biological Activity for Sulforhodamine 101
Sulforhodamine 101 is a fluorescent dye (excitation maximum ~ 586 nm; emission maximum ~ 606). Selective astrocyte marker.
Technical Data for Sulforhodamine 101
| M. Wt | 606.71 |
| Formula | C31H30N2O7S2 |
| Storage | Store at +4°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 60311-02-6 |
| PubChem ID | 122180 |
| InChI Key | COIVODZMVVUETJ-UHFFFAOYSA-N |
| Smiles | O=S(C(C=C8)=CC(S(=O)([O-])=O)=C8C(C(C=C7C6=C5CCCN6CCC7)=C5O4)=C(C4=C2CCC3)C=C1C2=[N+]3CCC1)(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Sulforhodamine 101
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| ethanol | 12.13 | 20 | |
| water | 30.34 | 50 |
Preparing Stock Solutions for Sulforhodamine 101
The following data is based on the product molecular weight 606.71. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.3 mL | 16.48 mL | 32.96 mL |
| 2.5 mM | 0.66 mL | 3.3 mL | 6.59 mL |
| 5 mM | 0.33 mL | 1.65 mL | 3.3 mL |
| 25 mM | 0.07 mL | 0.33 mL | 0.66 mL |
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Product Datasheets for Sulforhodamine 101
References for Sulforhodamine 101
References are publications that support the biological activity of the product.
Nimmerjahn et al (2004) Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat.Methods 1 31 PMID: 15782150
roth et al (2014) Transcranial amelioration of inflammation and cell death after brain injury. Nature 505 223 PMID: 24317693
If you know of a relevant reference for Sulforhodamine 101, please let us know.
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Keywords: Sulforhodamine 101, Sulforhodamine 101 supplier, specific, astrocyte, marker, astroglial, fluorescent, dye, labeling, labelling, labels, Labels, and, Stains, Neuron, Astrocyte, Probes, 5146, Tocris Bioscience
1 Citation for Sulforhodamine 101
Citations are publications that use Tocris products. Selected citations for Sulforhodamine 101 include:
Lezmy et al (2021) Astrocyte Ca 2+-evoked ATP release regulates myelinated axon excitability and conduction speed Science 374 eabh2858 PMID: 34648330
Do you know of a great paper that uses Sulforhodamine 101 from Tocris? Please let us know.
Reviews for Sulforhodamine 101
Average Rating: 5 (Based on 1 Review.)
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Selectively labels astrocytes.
By
Karthik Krishnamurthy on 11/15/2021
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: Cortical astrocytes
2 week old cultured cortical astrocytes labeled with 500 nM sulforhodamine 101 for 10 minutes.
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals