Ubiquitin-activating Enzyme E1

Ubiquitin-activating enzyme E1 catalyzes the first stage of the multistep enzymatic cascade of ubiquitination. Using ATP, the E1 enzyme binds to a ubiquitin molecule which is subsequently transferred to E2 enzymes, E3 enzymes, and then finally the target protein.

Products

Background

Literature (3)

Gene Data

Ubiquitin-activating Enzyme E1 Inhibitors
Cat. No.	Product Name / Activity
4993	NSC 624206
	Ubiquitin-activating enzyme (E1) inhibitor
2978	PYR 41
	Ubiquitin-activating enzyme (E1) inhibitor

Related Targets

Ubiquitin E3 Ligases

Ubiquitin-activating enzyme E1 catalyzes the first stage of the multistep enzymatic cascade of ubiquitination. The E1 enzyme binds to a ubiquitin molecule which is subsequently transferred to E2 enzymes, E3 enzymes, and then finally the target protein. Unlike the remainder of the ubiquitin-proteasome system, this enzymatic step is dependent on ATP.

There are currently two recognized E1 enzymes that catalyze the first stage of ubiquitination - UBA1 and UBA6 - with a further six related E1 enzymes identified that are involved in additional, ubiquitination-like processes such as SUMOylation and Neddylation. E1 enzymes are crucial for establishing specificity within the UPS, since they correctly match a ubiquitin molecule to an appropriate cognate E2 enzyme.

Studies on cells with mutations in UBA1 demonstrated the influence of E1 enzymes on cellular processes: UBA1 mutants displayed decreased cell proliferation and ubiquitin conjugation, and cell cycle arrest at the G₂/M phase. Endocytosis of growth hormone receptors was also shown to be abolished in E1 mutant cells, whilst conditional yeast E1 mutants display a defect in the Rad23-mediated delivery of target substrates to the proteasome, demonstrating the importance of E1 in a wide array of cellular processes.

External sources of pharmacological information for Ubiquitin-activating Enzyme E1 :

BJP Guide

Literature for Ubiquitin-activating Enzyme E1

Tocris offers the following scientific literature for Ubiquitin-activating Enzyme E1 to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Autophagy Scientific Review

Written by Patricia Boya and Patrice Codogno, this review summarizes the molecular mechanisms, physiology and pathology of autophagy. The role of autophagy in cell death and its links to disease are also discussed. Compounds available from Tocris are listed.

Autophagy Poster

Autophagy is a cellular process used by cells for degradation and recycling. Written by Patricia Boya and Patrice Codogno, this poster summarizes the molecular machinery, physiology and pathology of autophagy. Compounds available from Tocris are listed.

Parkinson's Disease Poster

Parkinson's disease (PD) causes chronic disability and is the second most common neurodegenerative condition. This poster outlines the neurobiology of the disease, as well as highlighting current therapeutic treatments for symptomatic PD, and emerging therapeutic strategies to delay PD onset and progression.