Proteasome

The proteasome complex is a broad spectrum protease present in all eukaryotes, which functions to carry out selective, efficient and progressive hydrolysis of intracellular target proteins. The proteasome plays a prominent role in the control of a diverse array of basic cellular processes by rapidly catalyzing numerous biological reactions.

The proteasome complex is composed of a 20S catalytic core and one or two terminal 19S regulatory units. The 20S catalytic domain is barrel-shaped and consists of seven structurally similar α- and β-subunits. In the inactive state, the external α-subunits act as a physical barrier to prevent peptide substrates accessing the active site on the β-subunits. The 19S regulatory units recognize proteins marked by polyubiquitin chains, remove the chain, entrap the protein moiety and open the 20S α-subunits allowing the β-subunits to degrade the protein. Assembly of the 20S domain is very complex and requires extrinsic (e.g. PAC1) and intrinsic (e.g. C-tail of β-subunits) chaperones. The assembly mechanism of the 19S domain is poorly understood, but Hsp90 is thought to play a role.

Regulation of the proteasome complex is mediated by proteasome interacting proteins (PIPs). There are two classes of PIPs; protein factors related to the ubiquitylation system such as the deubiquitinating enzyme, USP14, and auxillary factors that regulate proteasome functions via direct binding, for example Ecm29.

External sources of pharmacological information for Proteasome :

• BJP Guide

Literature for Proteasome

Tocris offers the following scientific literature for Proteasome to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

TPD and Induced Proximity Research Product GuideUpdated

This brochure highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation and Induced Proximity research, including:

• Active Degraders
• TAG Degradation Platform
• Degrader Building Blocks
• Assays for Protein Degradation
• Induced Proximity Tools

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Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia

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Proteasome Gene Data