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Submit ReviewApoptosis Activator 2
Description: Promotes apoptosome formation and activates caspase-9/caspase-3 pathway. Selectively induces tumor cell apoptosis
Chemical Name: 1-[(3,4-Dichlorophenyl)methyl]-1H-indole-2,3-dione
Purity: ≥99% (HPLC)
Biological Activity for Apoptosis Activator 2
Apoptosis Activator 2 is an apoptosis activator; promotes the cytochrome c-dependent oligomerization of Apaf-1 into the mature apoptosome. Increases procaspase-9 processing and subsequent caspase-3 activation. Induces apoptosis in tumor cells (IC50 = 4 - 9 μM for leukemia cells) with weak or no effect on normal cell lines or those defective/deficient in Apaf-1, caspase-9 or caspase-3 activity (IC50 > 40 μM).
Technical Data for Apoptosis Activator 2
| M. Wt | 306.14 |
| Formula | C15H9Cl2NO2 |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 79183-19-0 |
| PubChem ID | 1901244 |
| InChI Key | KGRJPLRFGLMQMV-UHFFFAOYSA-N |
| Smiles | ClC1=CC=C(CN2C(=O)C(=O)C3=CC=CC=C23)C=C1Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Apoptosis Activator 2
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| ethanol | 1.53 | 5 | |
| DMSO | 30.61 | 100 |
Preparing Stock Solutions for Apoptosis Activator 2
The following data is based on the product molecular weight 306.14. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.27 mL | 16.33 mL | 32.66 mL |
| 5 mM | 0.65 mL | 3.27 mL | 6.53 mL |
| 10 mM | 0.33 mL | 1.63 mL | 3.27 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.65 mL |
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Product Datasheets for Apoptosis Activator 2
References for Apoptosis Activator 2
References are publications that support the biological activity of the product.
Alavian et al (2009) Elevtaed p75NTR expression causes death of engrailed-deficient midbrain DArgic neurons by Erk1/2 suppression. Neural Dev. 4 11 PMID: 19291307
Jayaraman and Pike (2009) Progesterone attenuates oestrogen neuroprotection via downregulation of oestrogen receptor expression in cultured neurones. J.Neuroendocrinol. 21 77 PMID: 19094096
Nguyen and Wells (2003) Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc.Natl.Acad.Sci.USA 100 7533
If you know of a relevant reference for Apoptosis Activator 2, please let us know.
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Keywords: Apoptosis Activator 2, Apoptosis Activator 2 supplier, Promotes, apoptosome, formation, activates, caspase-9/caspase-3, pathway, induces, apoptosis, Selective, Inducer, Apoptosis, Activator2, Caspases, Inducers, 2098, Tocris Bioscience
2 Citations for Apoptosis Activator 2
Citations are publications that use Tocris products. Selected citations for Apoptosis Activator 2 include:
Pan et al (2016) SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Res 26 190 PMID: 26768768
Stankiewicz et al (2005) Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation. PLoS One 280 38729 PMID: 16172114
Do you know of a great paper that uses Apoptosis Activator 2 from Tocris? Please let us know.
Reviews for Apoptosis Activator 2
Average Rating: 5 (Based on 1 Review.)
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Apoptosis Activator 2 was used to induce apoptosis in epidermal cancer cell line: Works ideally.
By
Anonymous on 02/13/2019
Species: Human
Apoptosis Activator 2 was used to induce apoptosis in epidermal cancer cell line.Works great and gives publishable outcome.
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