Home / Product Type / Fluorescence Imaging / Fluorescent Probes and Stains / Other Fluorescent Probes / BI 2852 FL

BI 2852 FL

Cat. No. 8110 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Pan RAS Tracer
Chemical Name: N-(15-(5,5-Difluoro-7-(1H-pyrrol-2-yl)-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-13-oxo-3,6,9-trioxa-12-azapentadecyl)-5-(2-((6-((((3-(6-hydroxy-3-oxoisoindolin-1-yl)-1H-indol-2-yl)methyl)amino)methyl)-1H-indol-1-yl)methyl)-1H-imidazol-1-yl)pentanamide
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for BI 2852 FL

BI 2852 FL is a cell-permeable pan-RAS bioluminescence resonance energy transfer (BRET) fluorescent probe. BI 2852 FL is synthesized by conjugating a fluorophore to a derivative of the reversible SI/II-P KRAS inhibitor, BI 2852. BI 2852 FL is used in competitive BRET format to quantify SII-P engagement to multimeric RAS complexes in live cells.

Technical Data for BI 2852 FL

M. Wt 1088.04
Formula C59H64BF2N11O7
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2762758-95-0
PubChem ID 162640683
InChI Key VINGSJQDAYACET-UHFFFAOYSA-N
Smiles O=C1NC(C2=CC(O)=CC=C12)C3=C(NC4=C3C=CC=C4)CNCC5=CC6=C(C=C5)C=CN6CC7=NC=CN7CCCCC(NCCOCCOCCOCCNC(CCC8=CC=C9C=C%10C=CC(C%11=CC=CN%11)=[N+]%10[B-](N98)(F)F)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BI 2852 FL

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.88 10

Preparing Stock Solutions for BI 2852 FL

The following data is based on the product molecular weight 1088.04. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 9.19 mL 45.95 mL 91.91 mL
0.5 mM 1.84 mL 9.19 mL 18.38 mL
1 mM 0.92 mL 4.6 mL 9.19 mL
5 mM 0.18 mL 0.92 mL 1.84 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for BI 2852 FL

References are publications that support the biological activity of the product.

Vasta et al (2022) KRAS is vulnerable to reversible switch-II pocket engagement in cells. Nat.Chem.Biol. 18 596 PMID: 35314814

If you know of a relevant reference for BI 2852 FL, please let us know.

View Related Products by Target

Keywords: BI 2852 FL, BI 2852 FL supplier, BI2852FL, BI-2852, FL, fluorescent, probes, fluorescence, bioluminescence, resonance, energy, transfer, BRET, KRAS, Other, Fluorescent, Probes, Ras, GTPases, 8110, Tocris Bioscience

Citations for BI 2852 FL

Citations are publications that use Tocris products.

Currently there are no citations for BI 2852 FL. Do you know of a great paper that uses BI 2852 FL from Tocris? Please let us know.

Reviews for BI 2852 FL

There are currently no reviews for this product. Be the first to review BI 2852 FL and earn rewards!

Have you used BI 2852 FL?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including Janelia Fluor® Dyes
  • Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
  • Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
  • Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals
  • Tissue Clearing Kits and Reagents