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Submit ReviewBI dihydrochloride
Description: DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers
Chemical Name: (5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride
Purity: ≥98% (HPLC)
Biological Activity
BI is a DFHBI derivative for imaging of RNA in cells. BI increases brightness and photostability of Broccoli aptamers in cells. BI enables imaging of single Broccoli-tagged mRNAs in living cells. BI exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli. BI exhibits higher affinity for Broccoli aptamers and a 10.5-fold increase in cellular fluorescence compared with DFHBI-1T (Cat. No. 5610) (KD values are 51 and 305 nM, respectively).
Licensing Information
Sold under license from Lucerna
Optical Data for BI dihydrochloride
| Emission Color | Green |
|---|---|
| λabs | 470 nm |
| λem | 505 nm |
| Extinction Coefficient (ε) | 33600 M-1cm-1 |
| Quantum Yield (φ) | 0.669 |
| Cell Permeable | Yes |
| Application | DFHBI Derivative for Imaging of RNA in Living Cells |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
| M. Wt | 441.26 |
| Formula | C19H14F2N4O2.2HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 2758438-31-0 |
| InChI Key | YKXWKZLDMGAVJO-ZYMKCKPMSA-N |
| Smiles | CC1=N/C(C(N1CC2=C3NC=NC3=CC=C2)=O)=C\C4=CC(F)=C(C(F)=C4)O.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 44.13 | 100 | |
| DMSO | 22.06 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 441.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.27 mL | 11.33 mL | 22.66 mL |
| 5 mM | 0.45 mL | 2.27 mL | 4.53 mL |
| 10 mM | 0.23 mL | 1.13 mL | 2.27 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.45 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Li et al (2020) Fluorophore promoted RNA folding and photostability enables imaging of single Broccoli-tagged mRNAs in live mammalian cells. Angew.Chem.Int.Ed.Engl. 59 4511 PMID: 31850609
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals