Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewCaffeine Orange
Description: Selective, BODIPY-based fluorescent caffeine chemosensor
Chemical Name: (E)-5,5-Difluoro-3-(2-(5-fluoro-1H-indol-3-yl)vinyl)-1-methyl-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinine
Purity: ≥95% (HPLC)
Biological Activity for Caffeine Orange
Caffeine Orange is a selective, BODIPY-based fluorescent caffeine chemosensor, which exhibits up to 250-fold enhancement in its fluorescence upon caffeine activation (KD = 16.81 mM and detection limit of 50 µM). Caffeine Orange works both in aqueous and in dry state, and its red emission intensity increases with increasing caffeine concentrations. Excitation maximum ~580 nM; emission maximum ~629 nM.
Optical Data for Caffeine Orange
| Emission Color | Orange |
|---|---|
| λabs | 582 nm |
| λem | 618 nm |
| Extinction Coefficient (ε) | 70000 M-1cm-1 |
| Quantum Yield (φ) | 0.28 |
| Application | Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Caffeine Orange
| M. Wt | 365.17 |
| Formula | C20H15BF3N3 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 1632296-13-9 |
| PubChem ID | 86280912 |
| InChI Key | AROCVQXCAJKRTJ-GQCTYLIASA-N |
| Smiles | CC1=C2C=C3C=CC=[N+]3[B-](F)(N2C(/C=C/C4=CNC5=CC=C(C=C45)F)=C1)F |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Caffeine Orange
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 3.65 | 10 |
Preparing Stock Solutions for Caffeine Orange
The following data is based on the product molecular weight 365.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 27.38 mL | 136.92 mL | 273.85 mL |
| 0.5 mM | 5.48 mL | 27.38 mL | 54.77 mL |
| 1 mM | 2.74 mL | 13.69 mL | 27.38 mL |
| 5 mM | 0.55 mL | 2.74 mL | 5.48 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Caffeine Orange
References for Caffeine Orange
References are publications that support the biological activity of the product.
Xu et al (2013) Make caffeine visible: a fluorescent caffeine "traffic light" detector Sci.Rep. 3 2255 PMID: 23877095
If you know of a relevant reference for Caffeine Orange, please let us know.
View Related Products by Target
Keywords: Caffeine Orange, Caffeine Orange supplier, selective, fluorescent, caffeine, chemosensor, BODIPY, Other, Fluorescent, Probes, 7181, Tocris Bioscience
Citations for Caffeine Orange
Citations are publications that use Tocris products.
Currently there are no citations for Caffeine Orange. Do you know of a great paper that uses Caffeine Orange from Tocris? Please let us know.
Reviews for Caffeine Orange
There are currently no reviews for this product. Be the first to review Caffeine Orange and earn rewards!
Have you used Caffeine Orange?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals