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Submit ReviewD-Luciferin sodium salt
Description: Firefly luciferase substrate; cell permeable.
Chemical Name: (4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt
Purity: ≥99% (HPLC)
Biological Activity for D-Luciferin sodium salt
D-Luciferin sodium salt is a firefly luciferase substrate (Km = ~2 μM). Used in many standard bioluminescence imaging (BLI) techniques, including monitoring tumour growth, and investigating intracellular signaling activity in vitro and in vivo. Cell permeable. Emission maximum = 562 nm.
Optical Data for D-Luciferin sodium salt
| Emission Color | Green |
|---|---|
| λem | 562 nm |
| Cell Permeable | Yes |
| Application | Bioluminescence Imaging (BLI) Techniques, including monitoring tumor growth |
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Technical Data for D-Luciferin sodium salt
| M. Wt | 302.3 |
| Formula | C11H7N2NaO3S2 |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 103404-75-7 |
| PubChem ID | 23710675 |
| InChI Key | BZNVUYVALNTPBG-SREJTOIWSA-M |
| Smiles | OC1=CC2=C(N=C(C3=N[C@@H]([C@@]([O-])=O)CS3)S2)C=C1.[Na+] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for D-Luciferin sodium salt
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 30.23 | 100 | |
| DMSO | 30.23 | 100 |
Preparing Stock Solutions for D-Luciferin sodium salt
The following data is based on the product molecular weight 302.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.31 mL | 16.54 mL | 33.08 mL |
| 5 mM | 0.66 mL | 3.31 mL | 6.62 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.31 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
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Product Datasheets for D-Luciferin sodium salt
References for D-Luciferin sodium salt
References are publications that support the biological activity of the product.
Keyaerts et al (2008) Dynamic bioluminescence imaging for quantitative tumour burden assessment using IV or IP administration of D: -luciferin: effect on intensity, time kinetics and repeatability of photon emission. Eur.J.Nucl.Med.Mol.Imaging. 35 999 PMID: 18180921
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Keywords: D-Luciferin sodium salt, D-Luciferin sodium salt supplier, Firefly, luciferase, substrates, Biochemicals, and, Molecular, Biology, Bioluminescent, Substrates, 5427, Tocris Bioscience
Citations for D-Luciferin sodium salt
Citations are publications that use Tocris products.
Currently there are no citations for D-Luciferin sodium salt. Do you know of a great paper that uses D-Luciferin sodium salt from Tocris? Please let us know.
Reviews for D-Luciferin sodium salt
Average Rating: 4 (Based on 1 Review.)
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D-Luciferin was used to assess ATP availability in cellular assays.
By
Sam Sreshtha on 08/18/2019
Species: Human
D-Luciferin was used to assess ATP availability in cellular assays.The fluorescent images were distinguishable and publish worthy.
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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- Tissue Clearing Kits and Reagents
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