Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewDi 4 ANEPPS
Description: Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols
Chemical Name: 6-[2-(N,N-Dibutylamino)naphthyl]ethenyl-4'-pyridinium propanesulfonate
Purity: ≥90% (HPLC)
Biological Activity for Di 4 ANEPPS
Di 4 ANEPPS is a voltage-sensitive dye, also known as an electrochromic probe, which fluoresces in response to change in electrical potential across a cellular membrane. Di 4 ANEPPS fluoresces only when membrane bound; change in electrical potential causes an intramolecular charge transfer and blue-shift in the excitation spectra. Di 4 ANEPPS is commonly used to detect transient changes in membrane potential in excitable cells such as neurons and cardiac cells. Excitation ratio measurements can be used to determine membrane potential.
In model phospholipid membranes Ex/Em maxima are 465/635 nm, however spectral properties are highly dependent on membrane environment.
It is recommended to prepare stock solutions in DMSO.
Optical Data for Di 4 ANEPPS
| Emission Color | Red |
|---|---|
| λabs | 465 nm |
| λem | 635 nm |
| Application | Electrophysiology, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Di 4 ANEPPS
| M. Wt | 480.67 |
| Formula | C28H36N2O3S |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 90134-00-2 |
| PubChem ID | 6438327 |
| InChI Key | HAPJROQJVSPKCJ-UHFFFAOYSA-N |
| Smiles | [O-]S(CCC[N+](C=C1)=CC=C1/C=C/C(C=C2C=C3)=CC=C2C=C3N(CCCC)CCCC)(=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Di 4 ANEPPS
References for Di 4 ANEPPS
References are publications that support the biological activity of the product.
Loew et al (1992) A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J.Membr.Biol. 130 1 PMID: 1469705
Tominga & Tominga (2013) A new nonscanning confocal microscopy module for functional voltage-sensitive dye and Ca2+ imaging of neuronal circuit activity. J.Neurophysiol. 110 553 PMID: 23615547
Asamoah et al (2003) A fluorometric approach to local electric field measurements in a voltage-gated ion channel. Neuron. 37 85 PMID: 12526775
If you know of a relevant reference for Di 4 ANEPPS, please let us know.
View Related Products by Target
Keywords: Di 4 ANEPPS, Di 4 ANEPPS supplier, Di4ANEPPS, Di-4-ANEPPS, ANEP, dye, voltage, sensitive, probe, potentiometric, membrane, potential, electrochromic, electrophysiology, Fluorescent, Cell, Indicators, and, Sensors, 7324, Tocris Bioscience
Citations for Di 4 ANEPPS
Citations are publications that use Tocris products.
Currently there are no citations for Di 4 ANEPPS. Do you know of a great paper that uses Di 4 ANEPPS from Tocris? Please let us know.
Reviews for Di 4 ANEPPS
There are currently no reviews for this product. Be the first to review Di 4 ANEPPS and earn rewards!
Have you used Di 4 ANEPPS?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals