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Description: Fluorescent phosphatase substrate
Chemical Name: 6,8-Difluoro-4-methyl-7-(phosphonooxy)-2H-1-benzopyran-2-one
Purity: ≥95% (HPLC)
Biological Activity for DiFMUP
DiFMUP is a fluorescent phophatase substrate widely used for the detection of phosphatase activity. The reaction product of DiFMUP has excitation/emission maxima of 358/455 nm.
Note: This product is typically prepared in DMSO.
Technical Data for DiFMUP
| M. Wt | 292.13 |
| Formula | C10H7F2O6P |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 214491-43-7 |
| PubChem ID | 2786976 |
| InChI Key | DZANYXOTJVLAEE-UHFFFAOYSA-N |
| Smiles | CC1=CC(=O)OC2=C(F)C(O[P](O)(O)=O)=C(F)C=C12 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for DiFMUP
References for DiFMUP
References are publications that support the biological activity of the product.
Welte et al (2005) 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal.Biochem. 338 32 PMID: 15707933
Gee et al (1999) Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal.Biochem. 273 41 PMID: 10452797
If you know of a relevant reference for DiFMUP, please let us know.
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Citations for DiFMUP
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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