E 64d

Cat. No. 4545 6 Citations Submit a Review Datasheet / COA / SDS

Pricing	Availability		Qty

Description: Cathepsin inhibitor; interferes with autolysosomal digestion

Chemical Name: (2S,3S)-3-[[[(1S)-3-Methyl-1-[[(3-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]-2-oxiranecarboxylic acid ethyl ester

Purity: ≥98% (HPLC)

Datasheet

Citations (6)

Reviews

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for E 64d

E 64d is an inhibitor of cathepsins B and L; also thought to inhibit calpain. Inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with pepstatin A (Cat. No. 1190). Lysosome and cell permeable. Also inhibits entry of SARS-Cov-2 (COVID-19) into Caco-2 and Vero cells in combination with Camostat mesylate (Cat.No. 3193) in vitro.

Technical Data for E 64d

M. Wt	342.43
Formula	C₁₇H₃₀N₂O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	88321-09-9
PubChem ID	65663
InChI Key	SRVFFFJZQVENJC-IHRRRGAJSA-N
Smiles	O=[C@@](N[C@H]([C@](NCCC(C)C)=O)CC(C)C)[C@H]1O[C@@H]1C(OCC)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for E 64d

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	ethanol	6.85	20

Preparing Stock Solutions for E 64d

The following data is based on the product molecular weight 342.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	14.6 mL	73.01 mL	146.02 mL
1 mM	2.92 mL	14.6 mL	29.2 mL
2 mM	1.46 mL	7.3 mL	14.6 mL
10 mM	0.29 mL	1.46 mL	2.92 mL

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Product Datasheets for E 64d

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for E 64d

References are publications that support the biological activity of the product.

Kim et al (2008) Autophagy upregulation by inhibitors of caspase-3 and mTOR enhances radiotherapy in a mouse model of lung cancer. Autophagy 4 659 PMID: 18424912

Sato et al (2007) Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation. Cancer Res. 67 9677 PMID: 17942897

Tanada et al (2005) Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1 84 PMID: 16874052

Wilcox and Mason (1992) Inhibition of cysteine proteinases in lysosomes and whole cells. Biochem.J. 285 495 PMID: 1637341

Hoffmann et al (2020) SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically-proven protease inhibitor. Cell 181 271 PMID: 32142651

If you know of a relevant reference for E 64d, please let us know.

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