Home / Pharmacology / Enzymes / ATPases / H+-ATPase / H+-ATPase Activators / EN6

EN6

Cat. No. 7474 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: H+-ATPase activator; activates autophagy; inactivates mTORC1 signaling
Chemical Name: N-[4-Fluoro-3-[(1-oxo-2-propen-1-yl)amino]phenyl]-1-(2-fluorophenyl)-1H-pyrazole-4-carboxamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for EN6

EN6 is an activator of lysosomal vacuolar H+ ATPase (v-ATPase). EN6 covalently binds to the catalytic subunit of v-ATPase leading to increased acidification of lysosomes, inactivation of mTORC1 signaling and activation of autophagy in vivo. EN6 triggers entosis and promotes clearance of TDP43 protein aggregates in vitro.

Technical Data for EN6

M. Wt 368.34
Formula C19H14F2N4O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1808714-73-9
PubChem ID 99640033
InChI Key SUSXQEYPNDORDQ-UHFFFAOYSA-N
Smiles O=C(C=C)NC1=CC(=CC=C1F)NC(=O)C=2C=NN(C2)C=3C=CC=CC3F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for EN6

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.68 10

Preparing Stock Solutions for EN6

The following data is based on the product molecular weight 368.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 27.15 mL 135.74 mL 271.49 mL
0.5 mM 5.43 mL 27.15 mL 54.3 mL
1 mM 2.71 mL 13.57 mL 27.15 mL
5 mM 0.54 mL 2.71 mL 5.43 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for EN6

References are publications that support the biological activity of the product.

Chung et al (2019) Covalent targeting of the vacuolar H+-ATPase activates autophagy via mTORC1 inhibition. Nat.Chem.Biol. 15 776 PMID: 31285595

Su et al (2021) Role and dynamics of vacuolar pH during cell-in-cell mediated death. Cell Death Dis. 12 119 PMID: 33483474

If you know of a relevant reference for EN6, please let us know.

View Related Products by Target

View Related Products by Product Action

View all H+-ATPase Activators

Keywords: EN6, EN6 supplier, EN6, activators, activates, autophagy, mTORC1signaling, lysosomes, lysosomal, vacuolar, H+, ATPases, entosis, v-ATPase, mTORC1, Autophagy, H+-ATPase, 7474, Tocris Bioscience

Citations for EN6

Citations are publications that use Tocris products.

Currently there are no citations for EN6. Do you know of a great paper that uses EN6 from Tocris? Please let us know.

Reviews for EN6

There are currently no reviews for this product. Be the first to review EN6 and earn rewards!

Have you used EN6?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review