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Submit ReviewEvans Blue tetrasodium salt
Description: Dye for assessing cell viability and blood brain barrier permeability; also EAAT inhibitor and iGluR antagonist
Chemical Name: 6,6-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)bis[4-amino- 5-hydroxy-1,3-naphthalenedisulphonic acid] tetrasodium salt
Purity: ≥90% (HPLC)
Biological Activity for Evans Blue tetrasodium salt
Evans Blue tetrasodium salt is a potent inhibitor of L-glutamate uptake into synaptic vesicles. Also inhibits AMPA and kainate receptor-mediated currents (IC50 values are 220 and 150 nM respectively). P2X-selective purinoceptor antagonist. Also a non-permeating dye, stains damaged plasma membrane and enters in the cytoplasm and nucleus, used for checking cell viability.
Optical Data for Evans Blue tetrasodium salt
| Emission Color | Red |
|---|---|
| λem | 680 nm |
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Technical Data for Evans Blue tetrasodium salt
| M. Wt | 960.82 |
| Formula | C34H24N6Na4O14S4 |
| Storage | Store at RT |
| Purity | ≥90% (HPLC) |
| CAS Number | 314-13-6 |
| PubChem ID | 101102794 |
| InChI Key | ATNOAWAQFYGAOY-UHFFFAOYSA-J |
| Smiles | [Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1\N=N\C1=C(O)C2=C(C=C1)C(=CC(=C2N)S([O-])(=O)=O)S([O-])(=O)=O)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S([O-])(=O)=O)S([O-])(=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Evans Blue tetrasodium salt
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 9.61 | 10 |
Preparing Stock Solutions for Evans Blue tetrasodium salt
The following data is based on the product molecular weight 960.82. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 10.41 mL | 52.04 mL | 104.08 mL |
| 0.5 mM | 2.08 mL | 10.41 mL | 20.82 mL |
| 1 mM | 1.04 mL | 5.2 mL | 10.41 mL |
| 5 mM | 0.21 mL | 1.04 mL | 2.08 mL |
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Product Datasheets for Evans Blue tetrasodium salt
References for Evans Blue tetrasodium salt
References are publications that support the biological activity of the product.
Price and Raymond (1996) Evans blue antagonizes both α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate receptors and modulates desensitization. Mol. Pharmacol. 50 1665 PMID: 8967991
Roseth et al (1995) Uptake of L-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. J.Neurochem. 65 96 PMID: 7790899
Wittenburg et al (1996) P2-purinoceptor antagonists:II blockade of P2-purinoceptor subtypes and ecto-nucleotidase by compounds related to Evans blue and trypan blue. Naunyn Schmiedebergs Arch.Pharmacol. 354 491 PMID: 8897453
Schurmann et al (1997) Differential modulation of AMPA receptor mediated currents by Evans Blue in postnatal rat hippocampal neurones. Br.J.Pharmacol. 121 237 PMID: 9154333
Merck Index 12 3952
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2 Citations for Evans Blue tetrasodium salt
Citations are publications that use Tocris products. Selected citations for Evans Blue tetrasodium salt include:
Uderhardt et al (2019) Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell 177 541 PMID: 30955887
Gao et al (2018) Research articleHemolysis in human erythrocytes by Clostridium perfringens epsilon toxin requires activation of P2 receptors. Virulence 9 1601 PMID: 30277122
Do you know of a great paper that uses Evans Blue tetrasodium salt from Tocris? Please let us know.
Reviews for Evans Blue tetrasodium salt
Average Rating: 5 (Based on 1 Review.)
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Used for injection.
By
Anonymous on 06/06/2020
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: muscle
Evans blue solution 50 μl/g body weight injection to allow imaging of necrotic tissue in muscle dystrophy mouse model
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Fluorescent Dyes and Probes Research Product Guide
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