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Submit ReviewFluo-4 AM
Description: Cell-permeable, fluorescent Ca2+ indicator
Chemical Name: N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Biological Activity for Fluo-4 AM
Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.
It is recommended to prepare stock solutions in DMSO.
Optical Data for Fluo-4 AM
| Emission Color | Green |
|---|---|
| λabs | 494 nm |
| λem | 506 nm |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy, Flow Cytometry, Fluorometric Microplate Reading Assays |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Fluo-4 AM
| M. Wt | 1096.95 |
| Formula | C51H50F2N2O23 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 273221-67-3 |
| PubChem ID | 4060965 |
| InChI Key | QOMNQGZXFYNBNG-UHFFFAOYSA-N |
| Smiles | O=C(C(F)=C1)C=C2C1=C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C3)C5=CC(F)=C(OCOC(C)=O)C=C5O2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Fluo-4 AM
References for Fluo-4 AM
References are publications that support the biological activity of the product.
Gee et al (2000) Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes. Cell Calcium. 27 97 PMID: 10756976
If you know of a relevant reference for Fluo-4 AM, please let us know.
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Keywords: Fluo-4 AM, Fluo-4 AM supplier, Fluo-4AM, Fluo-4, cell, permeable, calcium, Ca2+, indicator, fluorescent, Ion, Indicators, General, Calcium, Signaling, Agents, Fluorescent, 6255, Tocris Bioscience
2 Citations for Fluo-4 AM
Citations are publications that use Tocris products. Selected citations for Fluo-4 AM include:
Bernardo et al (2020) Heterosynaptic Plasticity Determines the Set Point for Cortical Excitatory-Inhibitory Balance. Neuron 106 842-854.e4 PMID: 32213321
Gerald W et al (2022) Norepinephrine transporter defects lead to sympathetic hyperactivity in Familial Dysautonomia models. Nat Commun 13 7032 PMID: 36396637
Do you know of a great paper that uses Fluo-4 AM from Tocris? Please let us know.
Reviews for Fluo-4 AM
Average Rating: 4.5 (Based on 2 Reviews.)
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Fluo-4 detects store-operated Ca2+ entry upon ER Ca2+ store depletion in RBL-2H3 cells..
By
Niroj Shrestha on 12/24/2019
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: RBL-2H3
RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
works well in motor neurons.
By
Karthik krishnamurthy on 10/24/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: motor neurons
Used for imaging calcium entry in motor neurons at 1 mM concentration
motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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