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GF-AFC

Cat. No. 8143 Submit a Review Datasheet / COA / SDS
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Description: Cell permeable fluorogenic substrate cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays
Chemical Name: (2S)-2-[(2-Aminoacetyl)amino]-N-[2-oxo-4-(trifluoromethyl)chromen-7-yl]-3-phenylpropanamide hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for GF-AFC

GF-AFC (glycyl-phenylalanyl-aminofluorocoumarin) is a cell permeable peptide fluorogenic substrate. GF-AFC is cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays. GF-AFC can be used as a substrate for measuring the activity of dipeptidyl peptidase 1 (DPP1), an intracellular lysosomal cysteinyl protease.

Technical Data for GF-AFC

M. Wt 469.85
Formula C21H18F3N3O4.HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
InChI Key GBCCOIYISILSJP-NTISSMGPSA-N
Smiles NCC(N[C@@H](CC1=CC=CC=C1)C(NC2=CC=C(C(O3)=C2)C(C(F)(F)F)=CC3=O)=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for GF-AFC

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 46.98 100

Preparing Stock Solutions for GF-AFC

The following data is based on the product molecular weight 469.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.13 mL 10.64 mL 21.28 mL
5 mM 0.43 mL 2.13 mL 4.26 mL
10 mM 0.21 mL 1.06 mL 2.13 mL
50 mM 0.04 mL 0.21 mL 0.43 mL

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References for GF-AFC

References are publications that support the biological activity of the product.

Liu et al (2017) Serum based fluorescent assay for evaluating dipeptidyl peptidase I activity in collagen induced arthritis rat model. Mol.Cell.Probes 32 5 PMID: 27771442

Niles et al (2007) A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers. Anal.Biochem. 366 197 PMID: 17512890

If you know of a relevant reference for GF-AFC, please let us know.

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Citations for GF-AFC

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Currently there are no citations for GF-AFC. Do you know of a great paper that uses GF-AFC from Tocris? Please let us know.

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Literature in this Area

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