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Description: Fluorescent Ca2+ indicator
Chemical Name: 2-[4-[Bis[2-[(acetoxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid (acetoxy)methyl ester
Purity: ≥90% (HPLC)
Biological Activity for Indo 1AM
Indo 1AM is a fluorescent Ca2+ indicator (Kd for Ca2+ = 250 nM). Cell-permeable. Displays ratiometric emission wavelength change upon Ca2+ binding. Excitation/emission λ are 355/475 nm for no Ca2+ and 355/401 nm for high Ca2+. Absorption maxima are 349 and 331 nm for free anion and Ca complex, respectively. Respective emission maxima are 485 and 410 nm. Quantum yield ~0.5. Ideal for flow cytometry..
This product is typically prepared in DMSO.
Optical Data for Indo 1AM
| Emission Color | Blue |
|---|---|
| λabs | 355 nm |
| λem | 474 nm |
| Closest Laser line | 350 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Indo 1AM
| M. Wt | 1009.91 |
| Formula | C47H51N3O22 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 112926-02-0 |
| PubChem ID | 123918 |
| InChI Key | CAWBRCOBJNWRLK-UHFFFAOYSA-N |
| Smiles | CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=C(OCCOC2=C(C=CC(=C2)C2=CC3=C(N2)C=C(C=C3)C(=O)OCOC(C)=O)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=C(C)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Indo 1AM
References for Indo 1AM
References are publications that support the biological activity of the product.
Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314
If you know of a relevant reference for Indo 1AM, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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