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Submit ReviewJanelia Fluor® 525, NHS ester
Description: Yellow dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM. Suitable for live cell imaging
Alternative Names: JF 525,NHS
Chemical Name: 3,6-Di-1-(3,3-difluoroazetidinyl)-9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]xanthylium, inner salt
Biological Activity for Janelia Fluor® 525, NHS ester
Key information: Janelia Fluor® 525, NHS ester is a yellow fluorescent dye; supplied with an NHS ester reactive for the labeling of primary amines. Suitable for live cell imaging.
Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques, including dSTORM (in both live and fixed cells). Janelia Fluor® 525, NHS ester is cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 635 NHS ester (Cat. No. 6419). Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M-1cm-1; A280 correction factor = 0.185; lactone-zwitterion equilibrium constant (KL-Z) = 0.068.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 525, SE.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 525, NHS ester
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 525 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 122,000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.185 |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
| Alternative For | Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Janelia Fluor® 525, NHS ester
| M. Wt | 623.51 |
| Formula | C31H21F4N3O7 |
| Storage | Store at -20°C |
| CAS Number | 2975167-21-4 |
| PubChem ID | 134160239 |
| InChI Key | VPHPPEABRFKVDC-UHFFFAOYSA-N |
| Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 525, NHS ester
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 31.18 | 50 |
Preparing Stock Solutions for Janelia Fluor® 525, NHS ester
The following data is based on the product molecular weight 623.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.21 mL | 16.04 mL | 32.08 mL |
| 2.5 mM | 0.64 mL | 3.21 mL | 6.42 mL |
| 5 mM | 0.32 mL | 1.6 mL | 3.21 mL |
| 25 mM | 0.06 mL | 0.32 mL | 0.64 mL |
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Product Datasheets for Janelia Fluor® 525, NHS ester
References for Janelia Fluor® 525, NHS ester
References are publications that support the biological activity of the product.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 14 987 PMID: 28869757
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Janelia Fluor® 525, NHS ester, please let us know.
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Citations for Janelia Fluor® 525, NHS ester
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Protocols for Janelia Fluor® 525, NHS ester
The following protocol features additional information for the use of Janelia Fluor® 525, NHS ester (Cat. No. 6296).
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