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Submit ReviewPA Janelia Fluor® 646, Maleimide New
Description: Red photoactivatable dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Chemical Name: 3,7-Di(azetidin-1-yl)-2'-diazo-N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-5,5-dimethyl-3'-oxo-2',3'-dihydro-5H-spiro[dibenzo[b,e]siline-10,1'-indene]-6'-carboxamide
Purity: ≥90% (HPLC)
Biological Activity for PA Janelia Fluor® 646, Maleimide
Key information: PA Janelia Fluor® 646, Maleimide is a red cell-permeable photoactivatable fluorescent dye; supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live and fixed cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 649 - 651 nm and 663 - 665 nm, respectively.
Please see the product protocol for further information and a guide to protein/antibody labeling.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
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Technical Data for PA Janelia Fluor® 646, Maleimide
| M. Wt | 642.79 |
| Formula | C36H34N6O4Si |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | AUOMWYIWQORWPM-UHFFFAOYSA-N |
| Smiles | O=C(C1=CC=C(C(C23C4=CC=C(C=C4[Si](C)(C5=CC(N6CCC6)=CC=C35)C)N7CCC7)=C1)C(C2=[N+]=[N-])=O)NCCN8C(C=CC8=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for PA Janelia Fluor® 646, Maleimide
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.43 | 10 |
Preparing Stock Solutions for PA Janelia Fluor® 646, Maleimide
The following data is based on the product molecular weight 642.79. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 15.56 mL | 77.79 mL | 155.57 mL |
| 0.5 mM | 3.11 mL | 15.56 mL | 31.11 mL |
| 1 mM | 1.56 mL | 7.78 mL | 15.56 mL |
| 5 mM | 0.31 mL | 1.56 mL | 3.11 mL |
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Product Datasheets for PA Janelia Fluor® 646, Maleimide
References for PA Janelia Fluor® 646, Maleimide
References are publications that support the biological activity of the product.
Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID: 27776112
If you know of a relevant reference for PA Janelia Fluor® 646, Maleimide, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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