Janelia Fluor® 630b, NHS ester

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Description: Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM
Chemical Name: 2,5-Dioxopyrrolidin-1-yl 5,5-dimethyl-3,7-bis(3-(methylsulfonyl)azetidin-1-yl)-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews

Biological Activity for Janelia Fluor® 630b, NHS ester

Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.

Key Information: Janelia Fluor® 630b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for characterizing the physical properties of dense biomolecule condensates and complex macromolecular assemblies.

Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).

Properties and Photophysical Data: excitation and emission maxima (λ) are 654 nm and 671 nm, respectively.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 630b, NHS ester

Janelia Fluor<sup>®</sup> 630b, NHS ester Dye Spectra
λabs 654 nm
λem 671 nm

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Technical Data for Janelia Fluor® 630b, NHS ester

M. Wt 735.9
Formula C35H37N3O9S2Si
Storage Store at -20°C
Purity ≥90% (HPLC)
InChI Key BTRXQVXFPGRVHI-UHFFFAOYSA-N
Smiles C[Si]1(C2=CC(N3CC(S(C)(=O)=O)C3)=CC=C2C4(C5=CC=C(N6CC(C6)S(C)(=O)=O)C=C51)C7=C(CO4)C=CC(C(ON8C(CCC8=O)=O)=O)=C7)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Janelia Fluor® 630b, NHS ester

References for Janelia Fluor® 630b, NHS ester

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