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Submit ReviewJanelia Fluor® 635b, Maleimide New
Description: Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM
Chemical Name: N-(2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-3,7-bis(3-fluoroazetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxamide
Purity: ≥90% (HPLC)
Biological Activity for Janelia Fluor® 635b, Maleimide
Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.
Key Information: Janelia Fluor® 635b, Maleimide is a spontaneously blinking fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity) for the labeling of protein. Suitable to characterize the physical properties of dense biomolecule condensates and complex macromolecular assemblies.
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Properties and Photophysical Data: derived from Janelia Fluor® 635, Maleimide (Cat. No. 8027). Excitation and emission maxima (λ) are 651 nM and 667 nM, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Scientific Data
Super-Resolution Microscopy
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Application of Janelia Fluor® 635b, Maleimide. 3D SMLM map of a FUS coacervate aged for 12 h containing 20 nM FUSA2C-JF635b; inset shows brightfield image; scale bar = 4 μm; n = 31,123 total localizations; colors indicate localization density within a 100 nm radius sphere: black = 1; orange 2–10; green = 11–20; cyan = 21–40; magenta = 41–100. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 635b, Maleimide
| λabs | 651 nm |
|---|---|
| λem | 667 nm |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Janelia Fluor® 635b, Maleimide
| M. Wt | 640.76 |
| Formula | C35H34F2N4O4Si |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | CVIGZVOMUSQNLV-UHFFFAOYSA-N |
| Smiles | C[Si]1(C)C2=CC(N3CC(F)C3)=CC=C2C4(OCC5=C4C=C(C(NCCN6C(C=CC6=O)=O)=O)C=C5)C7=CC=C(N8CC(F)C8)C=C71 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Janelia Fluor® 635b, Maleimide
References for Janelia Fluor® 635b, Maleimide
References are publications that support the biological activity of the product.
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