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Submit ReviewJanelia Fluor® 635b, NHS ester New
Description: Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM
Chemical Name: 2,5-Dioxopyrrolidin-1-yl 3,7-bis(3-fluoroazetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate
Purity: ≥90% (HPLC)
Biological Activity for Janelia Fluor® 635b, NHS ester
Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.
Key Information: Janelia Fluor® 635b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for DNA labelling using amine-terminated oligonucleotides. Also used to label oligonucleotides for super-resolution assays of transposase-accessible chromatin (ATAC-SMLM).
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Properties and Photophysical Data: derived from Janelia Fluor® 635, SE (Cat. No. 6419). Excitation and emission maxima (λ) are 654 nM and 671 nM, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Scientific Data
Super-Resolution Microscopy
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Application of Janelia Fluor® 635b, NHS ester. SMLM RNA-FISH image of mouse embryo fibroblast cells expressing MS2 in the 3′UTR of the beta-actin gene and labeled with Janelia Fluor® 635b-oligonucleotide from Janelia Fluor® 635b, NHS ester; scale bar: 5 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149
Super-Resolution Microscopy
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Application of Janelia Fluor® 635b, NHS ester. Representative 3D ATAC–SMLM rendering of accessible chromatin localizations in wild-type mouse embryonic stem (mES) cells using oligonucleotide probes labeled with Janelia Fluor® 635b, NHS ester; the color-coded localization density was calculated with a canopy radius of 250 nm; scale bars: 2 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 635b, NHS ester
| λabs | 654 nm |
|---|---|
| λem | 671 nm |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Janelia Fluor® 635b, NHS ester
| M. Wt | 615.71 |
| Formula | C33H31F2N3O5Si |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | VNVYFQFCQSHREC-UHFFFAOYSA-N |
| Smiles | C[Si]1(C)C2=CC(N3CC(F)C3)=CC=C2C4(OCC5=C4C=C(C(ON6C(CCC6=O)=O)=O)C=C5)C7=CC=C(N8CC(F)C8)C=C71 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Janelia Fluor® 635b, NHS ester
References for Janelia Fluor® 635b, NHS ester
References are publications that support the biological activity of the product.
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