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Submit ReviewJanelia Fluor® 646b, Maleimide New
Description: Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM
Chemical Name: 3,7-Di(azetidin-1-yl)-N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxamide
Purity: ≥90% (HPLC)
Biological Activity for Janelia Fluor® 646b, Maleimide
Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.
Key Information: Janelia Fluor® 646b, Maleimide is a spontaneously blinking fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity) for the labeling of protein. Suitable to characterize the physical properties of dense biomolecule condensates and complex macromolecular assemblies.
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Properties and Photophysical Data: derived from Janelia Fluor® 646, Maleimide (Cat. No. 6590). Excitation and emission maxima (λ) are 662 nm and 674 nm, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646b, Maleimide
| λabs | 662 nm |
|---|---|
| λem | 674 nm |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Janelia Fluor® 646b, Maleimide
| M. Wt | 604.78 |
| Formula | C35H36N4O4Si |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 2634129-70-5 |
| InChI Key | IQYALKQSUGPKKJ-UHFFFAOYSA-N |
| Smiles | C[Si]1(C)C2=CC(N3CCC3)=CC=C2C4(OCC5=C4C=C(C(NCCN6C(C=CC6=O)=O)=O)C=C5)C7=CC=C(N8CCC8)C=C71 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Janelia Fluor® 646b, Maleimide
References for Janelia Fluor® 646b, Maleimide
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