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Submit ReviewJanelia Fluor® 669, Maleimide New
Description: Red dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-2-oxoethyl)thio)-3,5,6-trifluorobenzoate
Purity: ≥95% (HPLC)
Biological Activity for Janelia Fluor® 669, Maleimide
Key information: Janelia Fluor® 669, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 669 nm and 689 nm, respectively.
Please see the product protocol for further information and a guide to protein/antibody labeling.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 669, Maleimide
| Emission Color | Red |
|---|---|
| λabs | 669 nm |
| λem | 689 nm |
| Reactive Group | Maleimide |
| Reactivity | Thiols |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, dSTORM, STED, Flow Cytometry, Live-cell Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Janelia Fluor® 669, Maleimide
| M. Wt | 718.82 |
| Formula | C36H33F3N4O5SSi |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | FUZVUVRRODXAGE-UHFFFAOYSA-N |
| Smiles | C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(NCCN4C(C=CC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 669, Maleimide
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.19 | 10 |
Preparing Stock Solutions for Janelia Fluor® 669, Maleimide
The following data is based on the product molecular weight 718.82. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.91 mL | 69.56 mL | 139.12 mL |
| 0.5 mM | 2.78 mL | 13.91 mL | 27.82 mL |
| 1 mM | 1.39 mL | 6.96 mL | 13.91 mL |
| 5 mM | 0.28 mL | 1.39 mL | 2.78 mL |
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Product Datasheets for Janelia Fluor® 669, Maleimide
References for Janelia Fluor® 669, Maleimide
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Fluorescent Dyes and Probes Research Product Guide
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