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Submit ReviewJFX™ 554, Maleimide New
Description: Deuterated Janelia Fluor® dye (Reactive group: Maleimide). Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Chemical Name: 4-((2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)-2-(3-(pyrrolidin-1-ium-1-ylidene-d8)-6-(pyrrolidin-1-yl-d8)-3H-xanthen-9-yl)benzoate trifluoroacetate
Purity: ≥90% (HPLC)
Biological Activity for JFX™ 554, Maleimide
Key Information: JFX™ 554, Maleimide is a yellow fluorescent deuterated Janelia Fluor® dye; supplied with a Maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.
Application: Suitable for confocal microscopy, super-resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: JFX™ 554, Maleimide shows enhanced brightness, photostability and chromostability. Maleimide can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 554 nm and 576 nm, respectively; quantum yield = 0.8; extinction coefficient = 104,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 4.97.
Please see the product protocol for further information and a guide to protein/antibody labeling.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
JFX is a trademark owned by the Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for JFX™ 554, Maleimide
| λabs | 554 nm |
|---|---|
| λem | 576 nm |
| Extinction Coefficient (ε) | 104,000 M-1cm-1 |
| Quantum Yield (φ) | 0.8 |
| Reactive Group | Maleimide |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for JFX™ 554, Maleimide
| M. Wt | 734.78 |
| Formula | C35H16D16N4O6.CF3CO2H |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | ODPVJSHHEVQADP-CXZJOTLHSA-N |
| Smiles | [2H]C(C([2H])([2H])C([2H])([2H])C/1([2H])[2H])([2H])[N+]1=C2C=CC3=C(C4=CC(C(NCCN5C(C=CC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC=C(N7C([2H])([2H])C([2H])([2H])C([2H])([2H])C7([2H])[2H])C=C6OC3=C/2.FC(F)(C(O)=O)F |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for JFX™ 554, Maleimide
References for JFX™ 554, Maleimide
References are publications that support the biological activity of the product.
Grimm et al (2021) A general method to improve fluorophores using deuterated auxochromes. JACS 1 690 PMID: 34056637
If you know of a relevant reference for JFX™ 554, Maleimide, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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