Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewJFX™ 650, NHS ester New
Description: Deuterated Janelia Fluor® dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Chemical Name: 2-(5,5-Dimethyl-3-(pyrrolidin-1-ium-1-ylidene-d8)-7-(pyrrolidin-1-yl-d8)-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
Purity: ≥90% (HPLC)
Biological Activity for JFX™ 650, NHS ester
Key Information: JFX™ 650, NHS ester is a red fluorescent deuterated Janelia Fluor® dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.
Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: JFX™ 650, NHS ester shows enhanced brightness, photostability and chromostability. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 650 nm and 667 nm, respectively; quantum yield = 0.53; extinction coefficient = 17,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.015.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
JFX is a trademark owned by the Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for JFX™ 650, NHS ester
| λabs | 650 nm |
|---|---|
| λem | 667 nm |
| Extinction Coefficient (ε) | 17,600 M-1cm-1 |
| Quantum Yield (φ) | 0.53 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, dSTORM, STED, Flow Cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for JFX™ 650, NHS ester
| M. Wt | 637.86 |
| Formula | C35H19D16N3O6Si |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 2396479-84-6 |
| PubChem ID | 153290020 |
| InChI Key | OJCIIHUZGDFMHK-UDNRTFQASA-N |
| Smiles | [2H]C(C([2H])([2H])C([2H])([2H])C/1([2H])[2H])([2H])[N+]1=C2C=CC3=C(C4=CC(C(ON5C(CCC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC=C(N7C([2H])([2H])C([2H])([2H])C([2H])([2H])C7([2H])[2H])C=C6[Si](C)(C)C3=C/2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for JFX™ 650, NHS ester
References for JFX™ 650, NHS ester
References are publications that support the biological activity of the product.
Grimm et al (2021) A general method to improve fluorophores using deuterated auxochromes. JACS 1 690 PMID: 34056637
If you know of a relevant reference for JFX™ 650, NHS ester, please let us know.
View Related Products by Target
Keywords: JFX™ 650, NHS ester, JFX™ 650, NHS ester supplier, JFX650, SE, JFX650-NHS, ester, Janelia, Fluor, 650-SE, fluorescent, dyes, NHS, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, STED, deuterated, Amine, Reactive, Dyes, 8163, Tocris Bioscience
Citations for JFX™ 650, NHS ester
Citations are publications that use Tocris products.
Currently there are no citations for JFX™ 650, NHS ester. Do you know of a great paper that uses JFX™ 650, NHS ester from Tocris? Please let us know.
Reviews for JFX™ 650, NHS ester
There are currently no reviews for this product. Be the first to review JFX™ 650, NHS ester and earn rewards!
Have you used JFX™ 650, NHS ester?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals