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K 114

Cat. No. 3144 Submit a Review Datasheet / COA / SDS
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Description: Amyloid fibril-specific fluorescent probe. Used for: amyloid fibril detection. Application: fluorescence microscopy, confocal microscopy
Chemical Name: 4,4'-[(2-Bromo-1,4-phenylene)di-(1E)-2,1-ethenediyl]bisphenol
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for K 114

Key information: K 114 is a potent amyloid fibril-specific fluorescent probe (EC50 = 20 - 30 nM).

Used for: amyloid fibril detection.

Application: fluorescence microscopy, confocal microscopy.

Properties and Photophysical Data: K 114 exhibits minimal fluorescence in aqueous buffers and fluoresces brightly in the presence of Aβ (1-40), α-synuclein and tau in situ. K 114 fluorescence is ph-dependent. Excitation and emission maxima (λ) are 370nm and 450 nm, respectively, at pH 8.5; excitation and emission maxima (λ) are 395nm and 520 nm, respectively, at pH 10.5.

Technical Data for K 114

M. Wt 393.27
Formula C22H17BrO2
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 872201-12-2
PubChem ID 44828481
InChI Key OXPHQQMZTXMEGO-RJTULKDBSA-N
Smiles OC(C=C3)=CC=C3/C=C/C1=CC(Br)=C(/C=C/C2=CC=C(O)C=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for K 114

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 39.33 100
ethanol 19.66 50

Preparing Stock Solutions for K 114

The following data is based on the product molecular weight 393.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.54 mL 12.71 mL 25.43 mL
5 mM 0.51 mL 2.54 mL 5.09 mL
10 mM 0.25 mL 1.27 mL 2.54 mL
50 mM 0.05 mL 0.25 mL 0.51 mL

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Product Datasheets for K 114

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for K 114

References are publications that support the biological activity of the product.

Crystal et al (2003) A comparison of amyloid fibrillogenesis using the novel fluorescent compound K114. J.Neurochem. 86 1359 PMID: 12950445

LeVine (2005) Mechanism of Aβ(1-40) fibril-induced fluorescence of (trans-trans)-1-bromo-2,5-bis(4-hydroxystyryl)benene (K114). Biochemistry 44 15937 PMID: 16313197

Stepanchuk et al (2021) Complex photophysical properties of K114 make for a versatile fluorescent probe for amyloid detection. ACS Chem.Neurosci. 12 1273 PMID: 33705095

If you know of a relevant reference for K 114, please let us know.

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Keywords: K 114, K 114 supplier, Amyloid, fibril-specific, fluorescent, dye, Fluorescent, Probes, Beta, Precursor, Protein, APP, Secretase, β-Amyloid, beta-Amyloid, b-amyloid, Peptides, K114, amyloidbeta, amyloidb, amyloidβ, B, 3144, Tocris Bioscience

Citations for K 114

Citations are publications that use Tocris products.

Currently there are no citations for K 114. Do you know of a great paper that uses K 114 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Fluorescent Dyes and Probes Research Product Guide

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