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Description: Polarity sensitive lipid membrane fluorescent probe. Used for: imaging lipid rafts. Application: fluorescent microscopy
Chemical Name: 1-[6-(Dimethylamino)-2-naphthalenyl]-1-dodecanone
Purity: ≥95% (HPLC)
Biological Activity for Laurdan
Key information: Laurdan is a polarity sensitive fluorescent probe for imaging lipid rafts in membranes (also known as lipid microdomains). Suitable for live and fixed cells as well as in whole tissues imaging with multiphoton microscopy.
Used for: imaging lipid raft and cell membrane investigations.
Application: fluorescence microscope. Suitable for use with generalized polarization (GP) imaging and scanning fluorescence correlation spectroscopy (FCS).
Properties and Photophysical Data: When Laurdan is incorporated into membranes, its fluorescence spectra is sensitive to the physical state of the surrounding phospholipids. Excitation and emission maxima (λ) are 366 nm and 497 nm, respectively; quantum yield = 0.61; extinction coefficient = 19,500 M-1cm-1. In gel and liquid phase membranes, excitation and emission maxima (λ) are 440 nm and 490 nm, respectively.
Optical Data for Laurdan
| Emission Color | Blue |
|---|---|
| λabs | 366 nm |
| λem | 497 nm |
| Extinction Coefficient (ε) | 19500 M-1cm-1 |
| Quantum Yield (φ) | 0.61 |
| Application | Polarity Sensitive Fluorescent Probe for Lipid Raft and Cell Membrane Investigations |
| Alternative For | C-Laurdan, Di-4-ANEPPDHQ |
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Technical Data for Laurdan
| M. Wt | 353.55 |
| Formula | C24H35NO |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 74515-25-6 |
| PubChem ID | 104983 |
| InChI Key | JHDGGIDITFLRJY-UHFFFAOYSA-N |
| Smiles | CCCCCCCCCCCC(C1=CC2=CC=C(N(C)C)C=C2C=C1)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Laurdan
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 7.07 | 20 |
Preparing Stock Solutions for Laurdan
The following data is based on the product molecular weight 353.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 14.14 mL | 70.71 mL | 141.42 mL |
| 1 mM | 2.83 mL | 14.14 mL | 28.28 mL |
| 2 mM | 1.41 mL | 7.07 mL | 14.14 mL |
| 10 mM | 0.28 mL | 1.41 mL | 2.83 mL |
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Product Datasheets for Laurdan
References for Laurdan
References are publications that support the biological activity of the product.
Sanchez et al (2012) Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo. Proc.Natl.Acad.Sci.U.S.A. 109 7314 PMID: 22529342
Owen et al (2011) Quantitative imaging of membrane lipid order in cells and organisms. Nat.Protoc. 7 24 PMID: 22157973
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Keywords: Laurdan, Laurdan supplier, fluorescent, probe, imaging, lipid, rafts, membrane, polarity, multiphoton, microscopy, generalized, polarization, GP, and, scanning, fluorescence, correlation, spectroscopy, FCS, Fluorescent, Lipid, Probes, Cell, Membrane, Stains, 7275, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
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